AR09965PU-L Methionine aminopeptidase / MAP (1-264, His-tag)

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0.5 mg / €750.00
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Methionine aminopeptidase / MAP

Product Description for Methionine aminopeptidase / MAP

Methionine aminopeptidase / MAP.
Presentation: Purified

Properties for Methionine aminopeptidase / MAP

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Peptidase M
Presentation Purified
Molecular weight 31.5 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0AE18
Purity > 90 % pure by SDS-PAGE
Background Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of MAP contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings
  1. Bernier SG, Taghizadeh N, Thompson CD, Westlin WF, Hannig G. Methionine aminopeptidases type I and type II are essential to control cell proliferation. J Cell Biochem. 2005 Aug 15;95(6):1191-203. PubMed PMID: 15962312.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli map protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE
Molecular weight:
(284aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT
Purity:
>90% pure by SDS-PAGE
State:
Liquid purified protein
Purified
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