AR51921PU-N MIF (1-115, His-tag)

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0.5 mg / €730.00
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Quick Overview

MIF

Product Description for MIF

MIF.
Presentation: Purified

Properties for MIF

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Presentation Purified
Molecular weight 39.2 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14174
Purity > 90 % by SDS - PAGE
Background MIF also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Concentration 1.0 mg/ml (determined by bradford assay)
General Readings Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.Bucala R., et al. (1996) FASEB J 10: 1607-13.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human MIF, fused to GST-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFA MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA
Molecular weight:
(345aa) confirmed by MALDI-TOF
Format
Buffer System:
Phosphate Buffered Saline(pH7.4) containing 10% glycerol.
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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