PA1163XC Monokine induced by interferon-gamma (MIG)

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1 mg / €2,130.00

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Monokine induced by interferon-gamma (MIG)

Product Description for Monokine induced by interferon-gamma (MIG)

Monokine induced by interferon-gamma (MIG).
Presentation: Purified

Properties for Monokine induced by interferon-gamma (MIG)

Product Category Proteins & Growth Factors
Quantity 1 mg
Synonyms CXCL9
Presentation Purified
Molecular weight 12 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of Human MIG as a Reference Standard.
Background CXCL9 is a chemoattractant for activated T cells and possess angiostatic activity. Both CXCL9 and CXCL10 have been considered as important components for the anti-tumour activities of interferon-gamma (IFNgamma) and interleukin-12 in animal models. HuMIG is induced in THP-1 cells and in peripheral blood mononuclear cells by interferon-gamma but not by interferon-alpha or by lipopolysaccharide. Analysis of mouse and human genomic DNAs suggested that the Mumig and Humig genes are true mouse-human homologues.
Concentration 1 mg/ml
General Readings
  1. Egesten A, Eliasson M, Johansson HM, Olin AI, Morgelin M, Mueller A, et al. The CXC chemokine MIG/CXCL9 is important in innate immunity against Streptococcus pyogenes. J Infect Dis. 2007 Mar 1;195(5):684-93. Epub 2007 Jan 18. PubMed PMID: 17262710.
  2. Levy-Strumpf N, Culotti JG. VAB-8, UNC-73 and MIG-2 regulate axon polarity and cell migration functions of UNC-40 in C. elegans. Nat Neurosci. 2007 Feb;10(2):161-8. Epub 2007 Jan 21. PubMed PMID: 17237777.
  3. Wu M, Herman MA. Asymmetric localizations of LIN-17/Fz and MIG-5/Dsh are involved in the asymmetric B cell division in C. elegans. Dev Biol. 2007 Mar 15;303(2):650-62. Epub 2006 Dec 15. PubMed PMID: 17196955. (Free PMC Article available, 6 images available)
  4. Ranjbaran H, Wang Y, Manes TD, Yakimov AO, Akhtar S, Kluger MS, et al. Heparin displaces interferon-gamma-inducible chemokines (IP-10, I-TAC, and Mig) sequestered in the vasculature and inhibits the transendothelial migration and arterial recruitment of T cells. Circulation. 2006 Sep 19;114(12):1293-300. Epub 2006 Aug 28. PubMed PMID: 16940188.
  5. Zhang R, Tian L, Xiao F, Wen YJ, li J, Hou JM, et al. [Construction of pORF-mIG and activity evaluation of MIG in vitro]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jul;37(4):502-5. PubMed PMID: 16909588.
  6. Evidence that MIG-6 is a tumor-suppressor gene. Oncogene 2007 Jan 11;26(2):269-76
Storage Lyophilized MIG although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIG should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Human MIG (monokine induced by gamma-interferon ) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 103 amino acids.Recombinant Human MIG is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be, Thr-Pro-Val-Val-Arg.
Biological activity:
ED50 range= 10.0-100.0 ng/mL, determined by the dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2. The optimal concentration for each specific application should be determined by an initial dose response assay.
Molecular weight: 11700 Dalton.
Buffer System:
Lyophilized from a concentrated solution in water containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of Human MIG.
>98% Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized MIG in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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