AR50881PU-N mug (1-168, His-tag)

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0.25 mg / €730.00
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Quick Overview

mug

Product Description for mug

mug.
Presentation: Purified

Properties for mug

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms ECK3058, G/U mismatch-specific DNA glycosylase, JW3040, dug, xanthine DNA glycosylase, ygjF
Presentation Purified
Molecular weight 21.1 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A9H1
Purity > 90 % by SDS - PAGE
Background G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Lee HW., et al. (2010) J Biol Chem. 285(53):41483-90Gallinari P., et al. (1996) Nature. 383(6602):735-8.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R
Molecular weight:
(191aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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