AR50273PU-N mutM (1-269, His-tag)

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0.25 mg / €940.00
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Quick Overview

mutM

Product Description for mutM

mutM.
Presentation: Purified

Properties for mutM

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms Formamidopyrimidine-DNA glycosylase, fpg
Presentation Purified
Molecular weight 32.4 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05523
Purity > 90 % by SDS - PAGE
Background mutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings
  1. Serre L, Pereira de Jésus K, Boiteux S, Zelwer C, Castaing B. Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA. EMBO J. 2002 Jun 17;21(12):2854-65. PubMed PMID: 12065399. (Free PMC Article available, 10 images available)
  2. Schalow BJ, Courcelle CT, Courcelle J. Escherichia coli Fpg glycosylase is nonrendundant and required for the rapid global repair of oxidized purine and pyrimidine damage in vivo. J Mol Biol. 2011 Jul 8;410(2):183-93. doi: 10.1016/j.jmb.2011.05.004. Epub 2011 May 13. PubMed PMID: 21601577. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK
Molecular weight:
(289aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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