AR50880PU-N mutY (1-350, His-tag)

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50 µg / €730.00
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Quick Overview

mutY

Product Description for mutY

mutY.
Presentation: Purified

Properties for mutY

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB
Presentation Purified
Molecular weight 41.5 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17802
Purity > 90 % by SDS - PAGE
Background Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Lee H M., et al. (2004) J Neurochem. 88:394-400Tao H., et al. (2004) Carcinogenesis. 25:1859-1866.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant E.coil mutY protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV
Molecular weight:
(373aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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