AR50249PU-S ndk (1-143, His-tag)

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0.1 mg / €300.00
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ndk

Product Description for ndk

ndk.
Presentation: Purified

Properties for ndk

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms ECK2514, JW2502, Nucleoside diphosphate kinase
Presentation Purified
Molecular weight 18.0 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A763
Purity > 95 % by SDS - PAGE
Background ndk, also known as Nucleoside diphosphate kinase, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Lee B. et al. (2009) FEBS Lett. 583:3291-3295Kihara A. et al. (2011) Biosci Biotechnol Biochem, 75:1740-1745
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR
Molecular weight:
(167aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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