discontinued

AR01011PU-S Neuregulin 1 (beta-1 Isoform)

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Neuregulin 1

AR01011PU-S

Product Description for Neuregulin 1

Neuregulin 1.
Presentation: Purified

Properties for Neuregulin 1

Product Category Proteins & Growth Factors
Target Category
Quantity 10 µg
Synonyms Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, GGF, Glial growth factor, HGL, HRG, HRGA, Heregulin, NDF, NRG1, Neu differentiation factor, Pro-neuregulin-1 membrane-bound isoform, SMDF, Sensory and motor neuron-derived factor
Presentation Purified
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02297
Purity > 98 % > 98 % by SDS-PAGE gel and HPLC analyses
Background Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).
General Readings
  1. Wang L, Schulz TC, Sherrer ES, Dauphin DS, Shin S, Nelson AM, et al. Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood. 2007 Dec 1;110(12):4111-9. Epub 2007 Aug 29. PubMed PMID: 17761519. (Free PMC Article available, 5 images available)
  2. Lopez-Bendito G Tangential neuronal migration controls axon guidance: a role for neuregulin-1 in thalamocortical axon navigation. Cell.;125(1):127-42 4/7/2006 16615895.
Storage The lyophilized protein is stable at -20°C for one year from date of despatch.
Reconstituted Heregulin-β1 is stable for 3 months when stored in working aliquots with a carrier protein at -20°C.
Avoid repeated freezing and thawing.
Source E. coli
Description
Description:
Recombinant human Heregulin-β1 (HRG1-β1) is a polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).
AA Sequence:
SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
Biological activity:
The ED50 aas determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < / = 0.5 ng/ml.
Molecular weight:
7.5 kDa
Specific activity:
> / =2 x 10e6 units/mg
Format
Endotoxin level:
< 0.1 ng per μg (1EU/μg).
Purity:
>98% > 98 % by SDS-PAGE gel and HPLC analyses
State:
Sterile filtered, lyophilized, containing no additives
Purified
Reconstitution:
Centrifuge the vial prior to opening.
Restore in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 4°C for one week or at -20°C for future use.
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