AR10017PU-N Osteoprotegerin / TNFRSF11B (His-tagged)

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Osteoprotegerin / TNFRSF11B


Product Description for Osteoprotegerin / TNFRSF11B

Osteoprotegerin / TNFRSF11B.
Presentation: Purified

Properties for Osteoprotegerin / TNFRSF11B

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms OCIF, OPG, Osteoclastogenesis inhibitory factor, Tumor necrosis factor receptor superfamily member 11B
Presentation Purified
Molecular weight 31 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Swiss Prot Num:
Purity >80.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Application 1. Positive control for Western blot. 
2. Antibody production. 
3. Protein assay.

Osteoprotegerin, which is a member of the tumor necrosis factor receptor superfamily and is involved in the regulation of bone metabolism. OPGand its ligand (OPGL) are cytokines regulating osteoclasto-genesis. OPGL binds to receptors on the surface of preosteoclasts and stimulates their differentiation into active osteoclasts. This leads to osteoresorption. OPG inhibits this osteoclasto-genesis (OPG is secreted by osteoblasts, and binds to OPGL, thus inhibiting maturation of osteoclasts and osteoresorption). The degree and activity of osteoresorption depend mainly on the balance between OPG and its ligand (OPGL); factors increasing OPGL expression mostly reduce OPG expression and vice versa.

Storage Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OCIF should be stored at 2-8°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Source E. coli
Recombinant Human OCIF produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing amino acids 201-401 and having a molecular mass of 31 kDa which includes a 4 kDa His tag.
The OPG is purified by proprietary chromatographic techniques.
Buffer System:
Llyophilized from a concentrated (1 mg/ml) solution with 1x PBS, 0.1% SDS and 1mM DTT.
>80.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to restore in sterile 18MΩ-cm-1 H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
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