PA159X Osteoprotegerin / TNFRSF11B

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Osteoprotegerin / TNFRSF11B


Product Description for Osteoprotegerin / TNFRSF11B

Osteoprotegerin / TNFRSF11B.

Properties for Osteoprotegerin / TNFRSF11B

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms OCIF, OPG, Osteoclastogenesis inhibitory factor, Tumor necrosis factor receptor superfamily member 11B
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Swiss Prot Num:
Purity > 98 % pure by SDS-PAGE and HPLC analyses
Add. information Centrifuge the vial prior to opening!
Background Osteoprotegerin (OPG) is a member of the TNFR superfamily that can act as a decoy receptor for RANKL. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is secreted both as a monomeric and a dimeric protein. Its primary structure consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG.
General Readings
  1. Zhu, J. Implications for osteolytic bone metastases. The Journal of Biological Chemistry, 2007, 282(37):26656-64.
  2. Aoki K, Saito H, Itzstein C, Ishiguro M, Shibata T, Blanque R, et al. A TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption, and bone loss. J Clin Invest. 2006 Jun;116(6):1525-34. Epub 2006 May 4. PubMed PMID: 16680194. (Free PMC Article available, 8 images available)
  3. Frick KK, LaPlante K, Bushinsky DA. RANK ligand and TNF-alpha mediate acid-induced bone calcium efflux in vitro. Am J Physiol Renal Physiol. 2005 Nov;289(5):F1005-11. Epub 2005 Jun 21. PubMed PMID: 15972386.
  4. Sato N, Takahashi N, Suda K, Nakamura M, Yamaki M, Ninomiya T, et al. MyD88 but not TRIF is essential for osteoclastogenesis induced by lipopolysaccharide, diacyl lipopeptide, and IL-1alpha. J Exp Med. 2004 Sep 6;200(5):601-11. PubMed PMID: 15353553. (Free PMC Article available, 8 images available)
  5. Cheng X, Kinosaki M, Takami M, Choi Y, Zhang H, Murali R. Disabling of receptor activator of nuclear factor-kappaB (RANK) receptor complex by novel osteoprotegerin-like peptidomimetics restores bone loss in vivo. J Biol Chem. 2004 Feb 27;279(9):8269-77. Epub 2003 Dec 15. PubMed PMID: 14679212.
  6. Leslie DS, Vincent MS, Spada FM, Das H, Sugita M, Morita CT, et al. CD1-mediated gamma/delta T cell maturation of dendritic cells. J Exp Med. 2002 Dec 16;196(12):1575-84. PubMed PMID: 12486100. (Free PMC Article available, 7 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Recombinant Human OPG is a soluble 20.0 kDa protein containing 174 amino acid residues.
AA Sequence:
Biological activity:
Determined by its ability to inhibit TRAIL-induced apoptosis of LN-18 glioblastoma cells.
Molecular weight:
20.0 kDa
Buffer System:
10mM Sodium Phosphate, pH 7.5
Endotoxin level:
< 0.1 ng per µg (1EU/µg)
>98% pure by SDS-PAGE and HPLC analyses
Lyophilized (sterile filtered) purified fraction
Restore in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.
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