AR50868PU-S phrB (1-472, His-tag)

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20 µg / €300.00
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Quick Overview

phrB

Product Description for phrB

phrB.
Presentation: Purified

Properties for phrB

Product Category Proteins & Growth Factors
Quantity 20 µg
Synonyms Deoxyribodipyrimidine photolyase, ECK0697, JW0698, phrB
Presentation Purified
Molecular weight 56.1 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00914
Purity > 90 % by SDS - PAGE
Background Deoxyribodipyrimidine photolyase, also known as phrB, is involved in repair of UV radiation-induced DNA damage. This protein catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Concentration 0.25 mg/ml (determined by Bradford assay)
Product Citations
Purchased from Acris:
  1. Million-Weaver, SL. The consequences of head-on replication-transcription conflicts on replication restart and genomic instability in B. subtilis. Thesis 2015. https://digital.lib.washington.edu/researchworks/bitstream/handle/1773/34060/MillionWeaver_washington_0250E_14466.pdf?sequence=1
General Readings
  1. Byrdin M, Eker AP, Vos MH, Brettel K. Dissection of the triple tryptophan electron transfer chain in Escherichia coli DNA photolyase: Trp382 is the primary donor in photoactivation. Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8676-81. Epub 2003 Jun 30. PubMed PMID: 12835419. (Free PMC Article available, 3 images available)
  2. Weber S. Light-driven enzymatic catalysis of DNA repair: a review of recent biophysical studies on photolyase. Biochim Biophys Acta. 2005 Feb 25;1707(1):1-23. PubMed PMID: 15721603.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli phrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMTTHLVW FRQDLRLHDN LALAAACRNS SARVLALYIA TPRQWATHNM SPRQAELINA QLNGLQIALA EKGIPLLFRE VDDFVASVEI VKQVCAENSV THLFYNYQYE VNERARDVEV ERALRNVVCE GFDDSVILPP GAVMTGNHEM YKVFTPFKNA WLKRLREGMP ECVAAPKVRS SGSIEPSPSI TLNYPRQSFD TAHFPVEEKA AIAQLRQFCQ NGAGEYEQQR DFPAVEGTSR LSASLATGGL SPRQCLHRLL AEQPQALDGG AGSVWLNELI WREFYRHLIT YHPSLCKHRP FIAWTDRVQW QSNPAHLQAW QEGKTGYPIV DAAMRQLNST GWMHNRLRMI TASFLVKDLL IDWREGERYF MSQLIDGDLA ANNGGWQWAA STGTDAAPYF RIFNPTTQGE KFDHEGEFIR QWLPELRDVP GKVVHEPWKW AQKAGVTLDY PQPIVEHKEA RVQTLAAYEA ARKGK
Molecular weight:
(495aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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