discontinued

AR10191PU-N PIK3CD / PIK3R1 Heterodimer (active kinase)

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5 µg / €660.00

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PIK3CD / PIK3R1 Heterodimer

AR10191PU-N

Product Description for PIK3CD / PIK3R1 Heterodimer

PIK3CD / PIK3R1 Heterodimer.
Presentation: Purified

Properties for PIK3CD / PIK3R1 Heterodimer

Product Category Proteins & Growth Factors
Quantity 5 µg
Presentation Purified
Source Insect cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95.0% as determined by SDS Page
Storage

Store the antigen undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
The class IA PI3Kd is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3Kd in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3Kd in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein?s application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. 
The Molecular Weight for p110d chain is 120.2 kDa (without Tag) and the p85α chain 83.5 kDa.
Biological activity:
3,000 U/mg.
Unit Definition: 1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute.
Format
Buffer System:
25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2 and 50% Glycerol
Purity:
> 95.0% as determined by SDS Page
State:
Liquid sterile filtered solution
Purified
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