AR10718PU-S Polyphospate Kinase

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Polyphospate Kinase


Product Description for Polyphospate Kinase

Polyphospate Kinase.
Presentation: Purified

Properties for Polyphospate Kinase

Product Category Proteins & Growth Factors
Quantity 50 IU
Presentation Purified
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract


PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction. 

Storage Lyophilized PPK although stable at RT for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 2-8°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Please avoid freeze-thaw cycles. 
Shelf life: One year from despatch.
Source Propionibacterium shermanii
Polyphosphate Kinase purified circa 10 fold .
Free of all Polyphosphate Glucokinase activity.
Native Molecular Weight = 83 kD (monomer).
Biological activity:
The reaction requires Mg2+
The amount of Polyphosphate kinase required to convert 1μmole ADP to ATP per minute at pH 7.5, using Polyphosphate as phosphate donor.
Specific activity:
1.12 U/mg
Buffer System:
PPK solution containing 11.54 U/ml of PPK activity, 10.3 mg/ml total protein, 100mM Potassium Phosphate pH 6.8 and 25mM Sodium Polyphosphate.
Sterile filtered white lyophilized (freeze-dried) powder.
Restore in 1-10 ml deionized water
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