PA1267 Polyphosphate Glucokinase

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Polyphosphate Glucokinase


Product Description for Polyphosphate Glucokinase

Polyphosphate Glucokinase.
Presentation: Purified

Properties for Polyphosphate Glucokinase

Product Category Proteins & Growth Factors
Quantity 100
Presentation Purified
Molecular weight 63 kDa
Source P. shermanii
Specactivity 3.6 U/mg
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Background Polyphosphate Glucokinase catalyzes the phosphorylation of glucose using either inorganic polyphosphate or ATP as the phosphoryl donor. The phosphorylation of glucose plays a significant role in the creation of intermediates for oligosaccharide synthesis
General Readings
  1. Polyphosphate glucokinase. Prog Mol Subcell Biol 1999;23:101-25
  2. Hsieh PC, Kowalczyk TH, Phillips NF. Kinetic mechanisms of polyphosphate glucokinase from Mycobacterium tuberculosis. Biochemistry. 1996 Jul 30;35(30):9772-81. PubMed PMID: 8703950.
  3. Kowalczyk TH, Horn PJ, Pan WH, Phillips NF. Initial rate and equilibrium isotope exchange studies on the ATP-dependent activity of polyphosphate Glucokinase from Propionibacterium shermanii. Biochemistry. 1996 May 28;35(21):6777-85. PubMed PMID: 8639629.
  4. Hsieh PC, Shenoy BC, Samols D, Phillips NF. Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis. J Biol Chem. 1996 Mar 1;271(9):4909-15. PubMed PMID: 8617763.
  5. Kowalczyk TH, Phillips NF. Determination of endopolyphosphatase using polyphosphate glucokinase. Anal Biochem. 1993 Jul;212(1):194-205. PubMed PMID: 8396361.
  6. Kowalczyk TH, Szymona O. Glucose determination using immobilized polyphosphate glucokinase. Anal Biochem. 1991 Sep 2;197(2):326-32. PubMed PMID: 1664665.
Storage One year from despatch when stored at -20°C, three weeks at room temperature. Please avoid freeze-thaw cycles.
Source Propionibacterium shermanii
Polyphosphate Glucokinase purified circa 100 fold. Free of all polyphosphate kinase activity. Isoelectric point = 5.8.
Biological activity:
The specific activity is 3.6 U/mg.
Unit definition:
1 Unit of activity = the amount of PPGK required to convert 1 µmol glucose to glucose-6 phosphate per minute at 30°C and pH 7.4, in phosphate buffer using polyphosphate as phosphate donor.
Molecular weight:
Native MW = 63 kD, subunit MW = 30kD
Buffer System:
Contains 100 mM potassium phosphate, pH 6.8, and 25 mM sodium polyphosphate (Graham's salt)
Sterile filtered white lyophilized (freeze-dried) powder.
Reconstitute the lyophilized PPGK in 1-10 ml deionized water.
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