AR09117PU-N PPIL1 (1-166, His-tag)

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0.1 mg / €230.00
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PPIL1

AR09117PU-N

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  • AR09117PU-N

Product Description for PPIL1

PPIL1.
Presentation: Purified

Properties for PPIL1

Product Category Proteins & Growth Factors
Target Category
Quantity 0.1 mg
Synonyms CYPL1, PPIase, Peptidyl-prolyl cis-trans isomerase-like 1, Rotamase PPIL1
Presentation Purified
Molecular weight 19.3 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y3C6
Purity > 95 % by SDS PAGE
Background Peptidylprolyl isomerase (cyclophilin)-like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy.
Concentration 1.0 mg/ml (determined by Bradford assay)
Protocols Activity Assay
1.Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.
2.Add 10 ul of recombinant PPIL1 protein with 1 ug in assay buffer.
3.Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4.Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5.Record the increase in A405 nm for 30 minutes at 25°C.
General Readings Xu C., et al., (2006) J Biol Chem. 281(23):15900-8.
Obama K., et al., (2006) Clin Cancer Res. 12(1):70-6.
Storage Store (in aliquots) at -20°C or -70°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
AA Sequence:
MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH
Molecular weight:
(174 aa)
Specific activity:
> 300 nmoles/min/mg, defined as the amount of enzyme that cleaves
1 umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin
Format
Buffer System:
20 mM Tris-HCl pH 8.0, 20% glycerol
Purity:
>95% by SDS PAGE
State:
Liquid purified protein
Purified
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