RA100DL4 Protein A (DyLight405 conjugated)

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0.1 mg / €170.00

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Protein A (DyLight405 conjugated)


Product Description for Protein A (DyLight405 conjugated)

Protein A (DyLight405 conjugated).
Presentation: DyLight405

Properties for Protein A (DyLight405 conjugated)

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Presentation DyLight405
Species (Protein) S. aureus
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purity > 95 %
Application Western Blot: >1/20,000.
ELISA: >1/10,000.
Immunofluorescence: >1/5,000.
Background Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Single precipitin arc against anti-Protein A.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA and 0.01% (w/v) Sodium Azide
Label: DyLight(TM) 405 (MW 793)
Fluorochrome/Protein Ratio: 17.4 moles DyLight(TM) 405 per mole of Protein A
Absorption/Emission: 400 nm / 420 nm
Restore with 0.1 ml of deionized water (or equivalent).
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