discontinued

RA100DL5 Protein A (DyLight488 conjugated)

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0.1 mg / €170.00

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Protein A (DyLight488 conjugated)

RA100DL5

Product Description for Protein A (DyLight488 conjugated)

Protein A (DyLight488 conjugated).
Presentation: DyLight488

Properties for Protein A (DyLight488 conjugated)

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Presentation DyLight488
Species (Protein) S. aureus
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purity > 95 %
Application Western Blot: >1/20,000.
ELISA: >1/10,000.
Immunofluorescence: >1/5,000.
Background Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Single precipitin arc against anti-Protein A.
Format
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA and 0.01% (w/v) Sodium Azide
Label: DyLight(TM) 488 (MW 1,011.20)
Fluorochrome/Protein Ratio: 3,1 moles DyLight(TM) 488 per mole of Protein A
Absorption/Emission: 493 nm / 518 nm
Purity:
>95%
State:
Lyophilized
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
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