RA100D8 Protein A (IRDye800 conjugated)

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Protein A (IRDye800 conjugated)


Product Description for Protein A (IRDye800 conjugated)

Protein A (IRDye800 conjugated).
Presentation: IRDYE800

Properties for Protein A (IRDye800 conjugated)

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Presentation IRDYE800
Species (Protein) S. aureus
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Application Western Blot: 1/10,000 to 1/25,000.
Background Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the protein undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Single precipitin arc against anti-Protein A.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA and 0.01% (w/v) Sodium Azide
Label: IRDye(TM)800 (MW 1166.2)
Fluorochrome/Protein Ratio: 4.0 IRDye(TM)800 per mole of Protein A
Absorption/Emission: 778 nm / 806 nm
Restore with 0.5 ml of deionized water (or equivalent).
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