SA6016X Protein phosphatase 1A / PPM1A (1-382, His-tag)

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0.5 mg / €540.00
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Protein phosphatase 1A / PPM1A

Product Description for Protein phosphatase 1A / PPM1A

Protein phosphatase 1A / PPM1A.
Presentation: Purified

Properties for Protein phosphatase 1A / PPM1A

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms PP2C-alpha, PPPM1A, Protein phosphatase 2C isoform alpha, Protein phosphatase IA
Presentation Purified
Molecular weight 46.6 kDa
Source E. coli
Specactivity 8 kU/mg
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P35813
Purity > 95 % by SDS-PAGE
Background Protein phosphatase 2C (PP2Calpha) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Das, A. K. et al. (1996) EMBO J. 15, 6798-6809.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
The protein coding region of PP2Cα (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/HindШ site). PP2Cα was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
Molecular weight:
(418 aa), confirmed by MALDI-TOF
Specific activity:
Specific activity is > 1,400 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Format
Buffer System:
10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol
Endotoxin level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Purity:
>95% by SDS-PAGE
State:
Liquid purified protein
Purified
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