AR50127PU-N PSME3 (1-254, His-tag)

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0.25 mg / €730.00
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Quick Overview

PSME3

Product Description for PSME3

PSME3.
Presentation: Purified

Properties for PSME3

Product Category Proteins & Growth Factors
Target Category
Quantity 0.25 mg
Synonyms 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, PA28g, PA28gamma, Proteasome activator 28 subunit gamma, Proteasome activator complex subunit 3, REG-gamma
Presentation Purified
Molecular weight 31.7 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61289
Purity > 90 % by SDS - PAGE
Background PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage.
Concentration 0.5mg/ml (determined by Bradford assay)
General Readings Wilk S., et al. (2000) Arch. Biochem. Biophys. 383:265-271Zhang Z., et al. (2008) EMBO J. 27:852-864
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
Molecular weight:
(274aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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