AR50327PU-N pykF (1-470, His-tag)

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0.5 mg / €730.00
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Quick Overview

pykF

Product Description for pykF

pykF.
Presentation: Purified

Properties for pykF

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms ECK1672, JW1666, pyruvate kinase I
Presentation Purified
Molecular weight 53.3 kDa
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0AD61
Purity > 95 % by SDS - PAGE
Background pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis.
Concentration 1mg/ml (determined by Bradford assay)
General Readings Liapounova, Na, et al. (2006) Eukaryotic cell 5 (12): 2138-46,Valentini G., et al. (2000) J. Biol. Chem. 275:18145-18152
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL
Molecular weight:
(494aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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