AR51271PU-S RBM17 / SPF45 (1-401, His-tag)

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50 µg / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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RBM17 / SPF45

Product Description for RBM17 / SPF45

RBM17 / SPF45.
Presentation: Purified

Properties for RBM17 / SPF45

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms 45 kDa-splicing factor, RNA-binding motif protein 17, Splicing factor 45
Presentation Purified
Molecular weight 47.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96I25
Purity > 90 % by SDS - PAGE
Background RNA binding motif protein 17, also known as RBM17, is splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. This protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. This protein promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Lallena M.J., et al. (2002) Cell. 109:285-296
Carrascal M., et al. (2008) J. Proteome Res. 7:5167-5176
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant human RBM17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V
Molecular weight:
(421aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
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