discontinued

AR10430PU-S Resistin Like gamma

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5 µg / €230.00

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Resistin Like gamma

AR10430PU-S

Product Description for Resistin Like gamma

Resistin Like gamma.
Presentation: Purified

Properties for Resistin Like gamma

Product Category Proteins & Growth Factors
Quantity 5 µg
Presentation Purified
Molecular weight 11 kDa
Source E. coli
Species (Protein) Mouse
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q7TM98
Purity > 95.0% as determined by SDS-PAGE.
Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
Application ELISA. 
Western blotting.
Background

RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

Concentration 0.5 mg/ml (prior to lyophil.)
Storage Prior to reconstitution store at -20°C. 
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Mouse RELM-gamma is a His-Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues HisTag (underlined).
Specificity: The amino acid sequence of the RELM-g Mouse recombinant is 100% homologous to the amino acid sequence of the RELM-g Mouse.
AA Sequence:
MRGSHHHHHH GMASHM TLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA
Format
Buffer System:
0.05M Acetate buffer pH 4
Purity:
> 95.0% as determined by SDS-PAGE.
Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
State:
Sterile filtered lyophilized protein
Purified
Reconstitution:
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. 
For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/ml. In higher Concentrations the reconstitution of this antigen is limited.
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