Product Description for Ribonuclease HI / rnhA
Properties for Ribonuclease HI / rnhA
|Product Category||Proteins & Growth Factors|
|Synonyms||RNase H, RNase HI, Ribonuclease H, dasF, herA, rnh, sdrA|
|Species (Protein)||E. coli|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 95 %|
|Background||rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors.|
|Concentration||1.0 mg/ml (determined by Bradford assay)|
|General Readings||Nowotny M., et al. (2007) Mol Cell. 28(2):264-76.
Ten Asbroek AL., et al. (2002) Eur J Biochem. 269(2):583-92.
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.