AR39113PU-N Ribonuclease HI / rnhA (1-155, His-tag)

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0.1 mg / €300.00
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Ribonuclease HI / rnhA

AR39113PU-N

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  • AR39113PU-N

Product Description for Ribonuclease HI / rnhA

Ribonuclease HI / rnhA.
Presentation: Purified

Properties for Ribonuclease HI / rnhA

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms RNase H, RNase HI, Ribonuclease H, dasF, herA, rnh, sdrA
Presentation Purified
Molecular weight kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A7Y4
Purity > 95 %
Background rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Nowotny M., et al. (2007) Mol Cell. 28(2):264-76.
Ten Asbroek AL., et al. (2002) Eur J Biochem. 269(2):583-92.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant e.coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
Molecular weight:
(178aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Purity:
>95%
State:
Liquid purified protein
Purified
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