discontinued

PA1126XC Stem Cell Factor (SCF)

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1 mg / €2,130.00

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Stem Cell Factor (SCF)

PA1126XC

Product Description for Stem Cell Factor (SCF)

Stem Cell Factor (SCF).
Presentation: Purified

Properties for Stem Cell Factor (SCF)

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Molecular weight 18 kDa
Source E. coli
Species (Protein) Rat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE silver stained gel.
Endotoxin level is less than 0.1 ng/µg (IEU/µg) of SCF. Dimers/aggregates: less than 1%
General Readings 1. Neuroprotection by stem cell factor in rat cortical neurons involves AKT and NFkappaB. J Neurochem 2005 Oct;95(1):9-19
2. Quantification of stem cell factor mRNA levels in the rat testis: usefulness of clusterin mRNA as a marker of the amount of mRNA of Sertoli cell origin in post pubertal rats. J Endocrinol 2005 Jul;186(1):131-43
3. Activation of Akt (PKB) and suppression of FKHRL1 in mouse and rat oocytes by stem cell factor during follicular activation and development. Dev Biol 2005 May 15;281(2):160-70
4. Ghrelin inhibits the proliferative activity of immature Leydig cells in vivo and regulates stem cell factor messenger ribonucleic acid expression in rat testis.
Endocrinology 2004 Nov;145(11):4825-34
5. Survivin expression in rat testis is upregulated by stem-cell factor.
Mol Cell Endocrinol 2004 Apr 15;218(1-2):165-74
6. Stem cell factor and insulin-like growth factor-I stimulate luteinizing hormone-independent differentiation of rat ovarian
Storage Lyophilized Rat SCF, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freezing and thawing.
Source E. coli
Description
Description:
Recombinant Rat Stem Cell Factor (SCF) produced in E.Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Glu-Ile-Cys. Precursor- Protein structure and amino acid sequence: Format: This antigen is supplied as sterile filtered, white freeze-dried powder without additives, lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Tris pH-7.5 . It is recommended to reconstitute the Recombinant Rat Stem Cell Factor in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm 2. Analysis by RP-HPLC, using a calibrated solution of SCFas a Reference Standard.
Format
Purity:
>98% is greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE silver stained gel.
Endotoxin level is less than 0.1 ng/µg (IEU/µg) of SCF. Dimers/aggregates: less than 1%
State:
Purified
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