RA021C5 Streptavidin

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Product Description for Streptavidin


Properties for Streptavidin

Product Category Proteins & Growth Factors
Quantity 1 mg
Source S. avidinii
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purity Prepared from chromatographically pure Streptavidin.
Add. information Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays.
Recommended Dilutions:
Flow Cytometry: 1/200-1/400.
IF Microscopy: 1/100-1/1,000.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month several at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide as preservative.
Label: Conjugated to Cy5 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons).
Absorption/Emission Wavelength: 650 nm/667 nm
Fluorochrome/Protein Ratio: 7.1 moles Cy5 per mole of Streptavidin.
Prepared from chromatographically pure Streptavidin.
Lyophilized purifed Ig fraction.
Restore with 1.0 ml of deionized water (or equivalent).
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