PA1175 Stromal Cell Derived Factor-1 beta (SDF-1 beta)

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Stromal Cell Derived Factor-1 beta (SDF-1 beta)

Product Description for Stromal Cell Derived Factor-1 beta (SDF-1 beta)

Stromal Cell Derived Factor-1 beta (SDF-1 beta).
Presentation: Purified

Properties for Stromal Cell Derived Factor-1 beta (SDF-1 beta)

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms CXCL12, SDF1 beta
Presentation Purified
Molecular weight 9 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 98 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Add. information SDF-1b protein quantitation was carried out by two independent methods:
1. 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Human Stromal Cell-Derived Factor-1 beta as a Reference Standard.
Concentration 1.0 mg/ml
General Readings
  1. Gallagher KA, Liu ZJ, Xiao M, Chen H, Goldstein LJ, Buerk DG, et al. Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha. J Clin Invest. 2007 May;117(5):1249-59. PubMed PMID: 17476357. (Free PMC Article available, 7 images available)
  2. Massa A, Casagrande S, Bajetto A, Porcile C, Barbieri F, Thellung S, et al. SDF-1 controls pituitary cell proliferation through the activation of ERK1/2 and the Ca2+-dependent, cytosolic tyrosine kinase Pyk2. Ann N Y Acad Sci. 2006 Dec;1090:385-98. PubMed PMID: 17384283.
  3. Wysoczynski M, Kucia M, Ratajczak J, Ratajczak MZ. Cleavage fragments of the third complement component (C3) enhance stromal derived factor-1 (SDF-1)-mediated platelet production during reactive postbleeding thrombocytosis. Leukemia. 2007 May;21(5):973-82. Epub 2007 Mar 1. PubMed PMID: 17330096.
  4. Gao C, Li Y. SDF-1 plays a key role in the repairing and remodeling process on rat allo-orthotopic abdominal aorta grafts. Transplant Proc. 2007 Jan-Feb;39(1):268-72. PubMed PMID: 17275519.
  5. Verma R, Gupta RB, Singh K, Bhasin R, Anand Shukla A, Chauhan SS, et al. Distribution of CCR5delta32, CCR2-64I and SDF1-3'A and plasma levels of SDF-1 in HIV-1 seronegative North Indians. J Clin Virol. 2007 Mar;38(3):198-203. Epub 2007 Jan 19. PubMed PMID: 17240189.
  6. Fukui A, Goto T, Kitamoto J, Homma M, Asashima M. SDF-1 alpha regulates mesendodermal cell migration during frog gastrulation. Biochem Biophys Res Commun. 2007 Mar 9;354(2):472-7. Epub 2007 Jan 10. PubMed PMID: 17239342.
Storage Lyophilized SDF-1beta although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution SDF-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Source E.coli
Recombinant Human SDF-1b produced in E.Coli is a non-glycosylated, Polypeptide chain containing 72 amino acids.Human SDF-1b is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
Biological activity:
SDF-1b is fully biologically active when compared to standard.
The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.
Molecular weight: 8508 Dalton.
Buffer System:
SDF-1b is lyophilized from a concentrated sterile solution containing no additives.
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin level:
Less than 0.1 ng/µg (IEU/µg) of Recombinant Human Stromal Cell-Derived Factor-1 beta.
>98% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized SDF-1beta in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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