discontinued

AR10437PU-N Sulfate adenylyltransferase

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50 IU / €360.00

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Sulfate adenylyltransferase

AR10437PU-N

Product Description for Sulfate adenylyltransferase

Sulfate adenylyltransferase.
Presentation: Purified

Properties for Sulfate adenylyltransferase

Product Category Proteins & Growth Factors
Quantity 50 IU
Presentation Purified
Source E. coli
Species (Protein) Yeast
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P08536
Background

ATP sulphurylase synthesizes adenosine 5'-sul-phatophosphate from ATP and inorganic SO42-. This is the first reaction of a two step sequence in the formation of "active sulphate", adenosine 3'-phosphate5'-sulphatophosphate, which is a sulphate donor for a wide variety of compounds and is also involved in the reduction of sulphate.

Storage Prior to reconstitution store at 2-8°C for one month or desiccated below -18°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer. 
 For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Yeast Adenosine 5" Triphosphate Sulfurylase produced in E.coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Molecuar weight of 57.7 kDa.
Recombinant Yeast Adenosine 5" Triphosphate Sulfurylase catalyzes the activation of sulfate by transferring Sulfate to the Adenine monophosphate moiety of ATP to form Adenosine 5'-Phosphosulfate (APS) and Pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5 Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.
Unit Definition: One unit produces 1.0 µmole of ATP from APS and inorganic phosphate per min. at pH 8.0 and 30°C.
AA Sequence:
MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN GGFSPLTGFL NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT RIALFQDDEI PIAILTVQDV YKPNKTIEAE KVFRGDPEHP AISYLFNVAG DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR LEFQSRQWDR VVAFQTRNPM HRAHRELTVR AAREANAKVL IHPVVGLTKP GDIDHHTRVR VYQEIIKRYP NGIAFLSLLP LAMRMSGDRE AVWHAIIRKN YGASHFIVGR DHAGPGKNSK GVDFYGPYDA QELVESYKHE DIEVVPFRM VTYLPDEDRY APIDQIDTTK TRTLNISGTE LRRRLRVGGE IPEWFSYPEV VKILRESNPP RPKQGFSIVL GNSLTVSREQ LSIALLSTFL QFGGGRYYKI FEHNNKTELL SLIQDFIGSG SGLIIPNQWE DDKDSVVGKQ NVYLLDTSSS ADIQLESADE PISHIVQKVV LFLEDNGFFV F.
Biological activity:
12 Units/mg
Format
Buffer System:
Lyophilized after dialysis against lyophilized from 10mM NaP buffer, 100mM NaCl, 10mM Lactose, 1% PEG pH 7.5 and 0.75mM DTT.
State:
Sterile Filtered White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Spin vial before opening. Restore ATP sulphurylase with 5mM NaP pH-7.5 and 0.75mM DTT at a concentration ranging from 0.1-1 mg/ml.
Can be diluted further into other aqueous buffers. pH range between 7.0-8.5 is best.
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