PA161 TGFB1

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10 µg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

TGFB1

PA161

Product Description for TGFB1

TGFB1.
Presentation: Purified

Properties for TGFB1

Product Category Proteins & Growth Factors
Target Category
Quantity 10 µg
Synonyms TGF-beta-1, TGFB, Transforming growth factor beta-1
Presentation Purified
Molecular weight 25 kDa
Source CHO cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01137
Purity > 98 % pure by SDS-PAGE gel and HPLC analyses
Background The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.
General Readings
  1. Tartar DM, VanMorlan AM, Wan X, Guloglu FB, Jain R, Haymaker CL, et al. FoxP3+RORgammat+ T helper intermediates display suppressive function against autoimmune diabetes. J Immunol. 2010 Apr 1;184(7):3377-85. doi: 10.4049/jimmunol.0903324. Epub 2010 Feb 24. PubMed PMID: 20181889. (Free PMC Article available, 7 images available)
  2. Kwon HK, Lee CG, So JS, Chae CS, Hwang JS, Sahoo A, et al. Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2159-64. doi: 10.1073/pnas.0904055107. Epub 2010 Jan 13. PubMed PMID: 20080669. (Free PMC Article available, 7 images available)
  3. Wang J, Huizinga TW, Toes RE. De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid. J Immunol. 2009 Sep 15;183(6):4119-26. doi: 10.4049/jimmunol.0901065. Epub 2009 Aug 28. PubMed PMID: 19717521.
  4. Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, et al. Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology. Nat Protoc. 2009;4(2):232-43. doi: 10.1038/nprot.2008.229. PubMed PMID: 19197267.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Source made using a license from PHS, NIH, Patent Pending
Description
Description:
Recombinant Human TGF-beta-1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Biological activity:
The ED50 as determined by TGF-beta-1's ability to inhibit the Mouse IL-4-dependent proliferation of Mouse HT-2 cells is ≤ 0.05 ng/ml.
Specific activity:
≥ 2 x 107 units/mg
Format
Endotoxin level:
< 0.1 ng per µg (1 EU/µg)
Purity:
>98% pure by SDS-PAGE gel and HPLC analyses
State:
Lyophilized (0.2µ sterile filtered) purified protein without additives
Purified
Reconstitution:
Centrifuge the vial prior to opening and restore in water to a concentration of 0.1-1.0 mg/ml.
Do not vortex.
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