AR09459PU-N Thioredoxin-1 (TRX1) (2-109, His-tag)

Search for all "Thioredoxin-1 (TRX1)"

50 µg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Thioredoxin-1 (TRX1)

Product Description for Thioredoxin-1 (TRX1)

Thioredoxin-1 (TRX1).
Presentation: Purified

Properties for Thioredoxin-1 (TRX1)

Product Category Proteins & Growth Factors
Quantity 50 µg
Synonyms ECDH10B3970, TRX-1, TRXA, Thioredoxin 1
Presentation Purified
Molecular weight 12.9 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
B1X9Z5
Purity > 85 % by SDS – PAGE
Background Thioredoxin 1 (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Concentration 0.5 mg/ml (determined by Absorbance at 280nm)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli TXN protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS
Biological activity:
Specific activity is 16-18 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
2. Add 2 ug of recombinant E.coli thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes.
Molecular weight:
(119 aa), confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT
Purity:
>85% by SDS – PAGE
State:
Liquid purified protein
Purified
  • LinkedIn