discontinued

AR10713PU-N Thioredoxin

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20 µg / €300.00

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Thioredoxin

AR10713PU-N

Product Description for Thioredoxin

Thioredoxin.
Presentation: Purified

Properties for Thioredoxin

Product Category Proteins & Growth Factors
Quantity 20 µg
Presentation Purified
Source E. coli
Species (Protein) Yeast
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Anion-exchange FPLC. 
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Background Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. 
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
Recombinant Yeast Thioredoxin produced in E.coli is a single, non-glycosylated, polypeptide chain having a Molecular Mass of 12.6kDa
Biological activity:
Yeast Recombinant TRX is fully biologically active when compared to standard.
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM Bovine Insulin containing 0.33mM DTT (pH 6.5)
Specific activity:
3IU/mg
Format
Buffer System:
Each mg of protein contains 20mM Phosphate buffer pH 7.4
Purity:
> 95.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Anion-exchange FPLC. 
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
State:
Lyophilized sterile purified protein
Purified
Reconstitution:
Restore in sterile 18MΩ-cm-1 H2O
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