discontinued

AR10713PU-S Thioredoxin

Search for all "Thioredoxin"

5 µg / €230.00

Quick Overview

Thioredoxin

AR10713PU-S

Product Description for Thioredoxin

Thioredoxin.
Presentation: Purified

Properties for Thioredoxin

Product Category Proteins & Growth Factors
Quantity 5 µg
Presentation Purified
Source E. coli
Species (Protein) Yeast
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity > 95.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Anion-exchange FPLC. 
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Background Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. 
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description
Description:
Recombinant Yeast Thioredoxin produced in E.coli is a single, non-glycosylated, polypeptide chain having a Molecular Mass of 12.6kDa
Biological activity:
Yeast Recombinant TRX is fully biologically active when compared to standard.
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM Bovine Insulin containing 0.33mM DTT (pH 6.5)
Specific activity:
3IU/mg
Format
Buffer System:
Each mg of protein contains 20mM Phosphate buffer pH 7.4
Purity:
> 95.0% as determined by: 
(a) Analysis by RP-HPLC. 
(b) Anion-exchange FPLC. 
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
State:
Lyophilized sterile purified protein
Purified
Reconstitution:
Restore in sterile 18MΩ-cm-1 H2O
  • LinkedIn