discontinued

AR10127PU-N Thrombin

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1 mg / €750.00

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Thrombin

AR10127PU-N

Product Description for Thrombin

Thrombin.
Presentation: Purified

Properties for Thrombin

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Purity >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Background

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. 

Concentration 0.5 mg/ml (prior to lyophil.)
Storage Human Thrombin although stable at RT for 2-8°C, should be stored desiccated below -18°C. 
Avoid repeated freezing and thawing. 
Shelf life: One year from despatch.
Source Human Plasma
Description
Biological activity:
2000IU/mg.
Specific activity:
2000IU/mg.
Format
Buffer System:
The protein (0.5 mg/ml) was dialyzed against 5mM Sodium Citrate, 0.01% PEG, and 0.02M NaCl pH 6.5 before lyophilization.
Purity:
>95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
State:
Sterile white lyophilized powder.
Purified
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