Product Description for Thrombin
Properties for Thrombin
|Product Category||Proteins & Growth Factors|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Proteins (de)|
|Purity||> 95 % Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
|Background||Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.|
|General Readings||1. Complement inhibition attenuates brain edema and neurological deficits induced by thrombin. Acta Neurochir Suppl 2005;95:389-92
2. Development of nonobstructive intraarterial thrombi after injection of thrombin into pseudoaneurysms.
AJR Am J Roentgenol 2006 Feb;186(2):401-5
3. Activation of protein C by the thrombin-thrombomodulin complex: cooperative roles of Arg-35 of thrombin and Arg-67 of protein C.
Proc Natl Acad Sci U S A 2006 Jan 24;103(4):879-84
4. Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
Clin Pharmacol Ther 2006 Jan;79(1):23-34
5. Autoantibodies to thrombin directed against both of its cryptic exosites. Br J Haematol 2006 Feb;132(4):487-93
6. Thrombin and prothrombin are expressed by neurons and glial cells and accumulate in neurofibrillary tangles in Alzheimer disease brain. J Neuropathol Exp Neurol 2006 Jan;65(1):19-25
|Storage||HuThrombin although stable at room temperature for 4°C, should be stored desiccated
Please prevent freeze-thaw cycles.
Shelf life: one year from despatch.