GTX23828 Topo I marker for Sumoylation protein

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40 µl / €410.00

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Topo I marker for Sumoylation protein


Product Description for Topo I marker for Sumoylation protein

Topo I marker for Sumoylation protein.

Properties for Topo I marker for Sumoylation protein

Product Category Proteins & Growth Factors
Quantity 40 µl
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Purity n/a
Background This product can be used as part of an assay for sumoylation activity. Human Aos 1 + Uba 2 (GT23804), Ubc 9 (GT23803) and Sumo 1 (GT23801) can be used to promote in vitro sumoylation of a sumoylation marker (human Topoisomerase I protein fragment) (GT23828). The reaction products can be detected using our Sumo 1 (GT23819 and GT23824) and Topoisomerase I (GT23825) antibodies. Sumoylation assays are carried out in a final volume of 20 ul in reaction conditions (20 mM HEPES pH 7.5, 5mM MgCl2, 2mM ATP). Sumoylation Protocol: 1. Prepare a suitable purified substrate protein. (For the control, use 2ul Topoisomerase I marker for each reaction). 2. In each reaction, add 4ul E2 to substrate first, then 2ul Sumo 1, 2ul 10x reaction buffer, 2ul E1. Finally, add H2O to bring up to 20 ul. We would recommend adding fresh 2mM ATP to be sure that sufficient energy is supplied. 3. The best reaction concentration of proteins is as following: Aos 1 + Uba 2: 7.5ug/ml. Ubc 9: 50 ug/ml. SUMO 1: 50 ug/ml. For the control assay we recommend running the assay at 37
Storage Keep as concentrated solution. Store at 4
Buffer System:
PBS with 20% glycerol
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