AR19000PU-S Transglutaminase-2 (TGM2)

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Transglutaminase-2 (TGM2)


Product Description for Transglutaminase-2 (TGM2)

Transglutaminase-2 (TGM2).
Presentation: Purified

Properties for Transglutaminase-2 (TGM2)

Product Category Proteins & Growth Factors
Target Category
Quantity 5 µg
Synonyms Protein-glutamine gamma-glutamyltransferase 2, TGase C, TGase-H, Tissue transglutaminase
Presentation Purified
Molecular weight 78 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Swiss Prot Num:
Purity > 95 % pure as determined by SDS-PAGE
Application ELISA: Use at a concentration of 0.6-1.4 µg/ml (depending on the type of ELISA plate and coating buffer).
Western-Blot with Monoclonal anti-Hexa-His-tag antibody.
Suitable for Biotinylation and Iodination.
Immunological Functions:
1. Binds IgA & IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
Background Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.
Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.
Concentration 1 mg/ml
Storage Store the antigen at 2-8°C for 2-4 weeks or
frozen at -20°C for longer.
Avoid multiple freeze-thaw cycles.
Recombinant Human Tissue Transglutaminase is produced in E.coli is a non-glycosylated, polypeptide chain having a molecular mass of 78,018 Dalton. tTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.
Buffer System:
16mM HEPES buffer pH-8.0, 400mM NaCl and 20% Glycerol
>95% pure as determined by SDS-PAGE
Liquid purified protein
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