AR50332PU-N UBE2H (1-183, His-tag)

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0.5 mg / €730.00
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Quick Overview

UBE2H

Product Description for UBE2H

UBE2H.
Presentation: Purified

Properties for UBE2H

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms E2-20K, UBCH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2 H, Ubiquitin-protein ligase H
Presentation Purified
Molecular weight 23.1 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P62256
Purity > 95 % by SDS - PAGE
Background UBE2H (Ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2H is Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
Concentration 1mg/ml (determined by Bradford assay)
General Readings Kaiser P., et al. (1994) J. Biol. Chem. 269:8797-8802David Y., et al. (2010) J. Biol. Chem. 285:8595-8604
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human UBE2H protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL
Molecular weight:
(206aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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