AR50427PU-N UBE2M (1-183, His-tag)

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0.5 mg / €730.00
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Quick Overview

UBE2M

Product Description for UBE2M

UBE2M.
Presentation: Purified

Properties for UBE2M

Product Category Proteins & Growth Factors
Target Category
Quantity 0.5 mg
Synonyms NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC12, Ubiquitin-conjugating enzyme E2 M
Presentation Purified
Molecular weight 23.5 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61081
Purity > 95 % by SDS - PAGE
Background UBE2M, also known as NEDD8-conjugating enzyme Ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
Concentration 1 mg/ml (determined by Bradford assay)
General Readings Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042Huang D.T., et al. (2004) Nat. Struct. Mol. Biol. 11:927-935
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human UBE2M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK
Molecular weight:
(207aa) confirmed by MALDI-TOF
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT
Purity:
>95% by SDS - PAGE
State:
Liquid purified protein
Purified
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