NB800-PC41 Ubiquitin-AMC, Human Recombinant Protein

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Ubiquitin-AMC, Human Recombinant Protein


Product Description for Ubiquitin-AMC, Human Recombinant Protein

Ubiquitin-AMC, Human Recombinant Protein.

Properties for Ubiquitin-AMC, Human Recombinant Protein

Product Category Proteins & Growth Factors
Quantity 0.05 mg
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Substrate concentrations for a typical assay range from 0.1-1uM. Typical enzyme concentrations for UCH-L3 are 10 - 100 pM and for Isopeptidase-T are 10 - 100nM. Release of AMC fluorescence by UCH
enzymes can be monitored using wavelengths EX 380nm and EM 460nm, respectively.
Background Ubiquitin-AMC is purified human-recombinant ubiquitin derivatized at the C-terminus with 7-amido-4-methylcoumarin (AMC). Its use is as a fluorogenic substrate for Ubiquitin C-terminal Hydrolases (UCHs). It is an extremely sensitive substrate for UCH-L3 (Km = 0.039 uM) and for Isopeptidase-T (Km = 0.17 - 1.4 uM).
General Readings
  1. 1987. Annu. Rev. Cell Biol. 3:1-30.
  2. 1999. Annu. Rev. Med. 50:57-74.
  3. 1999. Biochem. 68:1015-1068.
  4. Dang, L.C., et al. 1998. Biochem. 37:1868.
Storage Store at -80C. Avoid freeze-thaw cycles.
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