NB800-PC39 Ubiquitin-(His)6, Human Recombinant Protein

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Ubiquitin-(His)6, Human Recombinant Protein


Product Description for Ubiquitin-(His)6, Human Recombinant Protein

Ubiquitin-(His)6, Human Recombinant Protein.

Properties for Ubiquitin-(His)6, Human Recombinant Protein

Product Category Proteins & Growth Factors
Quantity 2 mg
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Working concentrations for non-rate-limiting conjugate formation are 200 uM to 1 mM depending upon conditions.
Background (His)6-Ubiquitin allows for single-step purification of conjugates using nickel affinity resins (that is, metal-chelate affinity purification). Also allows for immuno-detecting ubiquitinated proteins using (His)6-specific antibodies. Supports proteasomal degradation similar to wild-type ubiquitin. Fully functional N-terminal His-tagged ubiquitin.
General Readings
  1. DiStefano, D.L. and Wand, J.A. 1987. Biochemistry. 26: 7272-7281.
  2. Beers, E.P. and Callis, J. 1993. J. Biol. Chem. 268: 21645-21649.
  3. Treier, M., et al. 1994. Cell. 78: 787-798.
Storage Store at -80C. Avoid freeze-thaw cycles.
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