discontinued

AR10156PU-L Urate Oxidase

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1 mg / €330.00

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Urate Oxidase

AR10156PU-L

Product Description for Urate Oxidase

Urate Oxidase.
Presentation: Purified

Properties for Urate Oxidase

Product Category Proteins & Growth Factors
Quantity 1 mg
Presentation Purified
Source E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q00511
Purity >96.0% as determined by both by RP-HPLC and SDS-PAGE analysis.
Background

Urate oxidase catalyzes the enzymatic oxidation (degrades) of uric acid into allantoin, an inactive and soluble metabolite, which is 5 to 10 fold more soluble than uric acid . Urate oxidase is an enzyme of the purine breakdown pathway that catalyses the oxidation of uric acid to allantoin. Uricase is present in numerous diverse organisms, but not in higher primates including Human Hyperuricaemia is most commonly associated with gout and also occurs in mammalians with malignancy, especially those with lymphoid malignancies due to rapid cell turnover and an increased rate of purine metabolism. Uricase is effective in the prevention and treatment of hyperuricaemia in mammalians with malignancy and in those who have undergone transplantation. It appears to act rapidly, safely and induces a more dramatic decrease in plasma levels of uric acid.

Storage Lyophilized Urate Oxidase although stable at RT for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution store the antibody undiluted at 2-8°C for 2-7 days or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant Human Urate Oxidase produced in E.coli is a tetrameric, non-glycosylated polypeptide chain containing 302 amino acids, having a Molecular formula of C1523H2383N417O462S7 and a Molecular Mass of 34,247 Dalton.
The cDNA coding for Urate-Oxidase was cloned from a strain of Aspergillus flavus .
The monomer protein has no intra- or inter-disulfide bridges.
AA Sequence:
msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl
Biological activity:
The specific activity was found to be 10U/mg.
One Unit oxidizes one micromole of uric acid per minute at 25°C, at pH 8.5.
Format
Buffer System:
Each 1.5 mg Uricase contains 5 mg Sucrose, 25 mg Glycine, 0.1 mg Tween-80, 13.6 mg Na2HPO4*12H20 and 0.33 mg NaH2PO4*2H20
Purity:
>96.0% as determined by both by RP-HPLC and SDS-PAGE analysis.
State:
Sterile Filtered White lyophilized (freeze-dried) powder
Purified
Reconstitution:
Restore in 50mM Borate buffer containing 0.001%Triton X-100 and 1.0mM EDTA, pH 8.5 for activity assay.
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