AR51176PU-S VPS4B (1-444, His-tag)

Search for all "VPS4B"

50 µg / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

VPS4B

Product Description for VPS4B

VPS4B.
Presentation: Purified

Properties for VPS4B

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms SKD1, Suppressor of K(+) transport growth defect 1, VPS42, Vacuolar protein sorting-associated protein 4B
Presentation Purified
Molecular weight 51.8 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O75351
Purity > 90 % by SDS - PAGE
Background Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding.
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Scheuring S., et al. (2001) J Mol Biol. 312:469-480. Perez O D., et al. (2001) Immunity. 15:687-690
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description
Description:
Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG
Molecular weight:
(468aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Purity:
>90% by SDS - PAGE
State:
Liquid purified protein
Purified
  • LinkedIn