AR50378PU-N YARS2 (17-477, His-tag)

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0.1 mg / €730.00
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Quick Overview

YARS2

Product Description for YARS2

YARS2.
Presentation: Purified

Properties for YARS2

Product Category Proteins & Growth Factors
Target Category
Quantity 0.1 mg
Synonyms CGI-04, TyrRS, Tyrosine-tRNA ligase, Tyrosyl-tRNA synthetase mitochondrial
Presentation Purified
Molecular weight 53.7 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9Y2Z4
Purity > 85 % by SDS - PAGE
Background YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Concentration 0.25 mg/ml (determined by Bradford assay)
General Readings Bonnefond L., et al. (2005) Biochemistry. 44:4805-4816Riley L.G., et al. (2010) Am. J. Hum. Genet. 87:52-59
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human YARS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL
Molecular weight:
(482aa)
Format
Buffer System:
20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Purity:
>85% by SDS - PAGE
State:
Liquid purified protein
Purified
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