Antibodies to ATPase Enzymes - Product Review 12

Introduction

ATPase proteins are enzymes which are able to supply energy by cleaving ATP into ADP and phosphate. This energy can be used for transport of ions into or out of the cell against a concentration gradient. There are several forms of membrane bound ATPase enzymes responsible for export or import of different ions.

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

The hydrogen/potassium ATPase, or gastric proton pump, belongs to a family of P-type cation-transporting ATPases. This family of ATPases shares a number of functional and structural similarities including the common feature of consisting of an alpha and beta subunit. Like the ubiquitous sodium/potassium ATPase, the hydrogen/potassium ATPase consists of a large transmembrane catalytic subunit, termed the alpha- subunit which contains sites for ATP binding and phosphorylation, and an associated smaller glycoprotein, termed the beta-subunit which may play a role in maintaining the structural and functional integrity of the complex.

Antibody Tools for Detection of Membrane Bound ATPase Enzymes

Acris Antibodies offers a wide range of antibodies specific for the different types of membrane bound ATPase enzymes and their respective isoforms. Monoclonal and polyclonal antibodies useful for multiple applications are listed in the table below. Species reactivity is broad in most cases due to high conservation of these proteins. Pricing and product informations are available using the links provided.

Available products

ATPase, PMCA, SERCA antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ATP1A3 antibody

ATP1A3 Mouse IgM 1D2 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP1B1 antibody

Detection of ATP1B1 in rat kidney homogenates ( 20 µg ) using ATP1B1 monoclonal antibody, clone 464.8 (also known as 8A) ( Cat # MAB2323 ).
Lane 1, 1 : 2,500 primary antibody.
Lane 2, 1 : 5,000 primary antibody. Mouse IgG2a 464.8 (also known as 8A) Bov, Hu, Rb, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ATP1B1 antibody

Western blot analysis detecting ATP1B1 in porcine proximal tubule protein, using ATP1B1 monoclonal antibody, clone 464.6 ( Cat # MAB2407 ). Mouse IgG1 464.6 Hu, Ms, Rb, Rt, Sh, Xen C, ICC/IF, WB
50 µl / €470.00
  Abnova Taiwan Corp.

ATP1B1 antibody

Human Heart; ATP1B1 antibody - middle region (ARP44411_P050) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

ATP1B2 antibody

Detection limit for recombinant GST tagged ATP1B2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 4E3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 4B8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 3H4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A1 / SERCA1 antibody

ATP2A1 / SERCA1 Mouse IgG3 2C9 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

ATP2A3 / SERCA3 antibody

ATP2A3 / SERCA3 Mouse IgG2a 1B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP2A3 / SERCA3 antibody

Human MCF7; Host:Rabbit. Target Name:ATP2A3. Sample Tissue:MCF7. Antibody Dilution: 1.0ug/ml. ATP2A3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.; ATP2A3 antibody - middle region (ARP46580_P050) in Human MCF7 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2B3 / PMCA3 antibody

Human Heart; WB Suggested Anti-ATP2B3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; ATP2B3 antibody - N-terminal region (ARP44390_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2B3 / PMCA3 antibody

Human Placenta; WB Suggested Anti-ATP2B3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; ATP2B3 antibody - N-terminal region (ARP44391_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ATP2C1 antibody

Immunofluorescence of purified MaxPab antibody to ATP2C1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ATP2C1 antibody

Immunofluorescence of purified MaxPab antibody to ATP2C1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATP2C1 antibody

ATP2C1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ATP2C1 expression in mouse testis. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ATP4B antibody

Western Blot analysis of ATP4B expression in transfected 293T cell line (H00000496-T01) by ATP4B MaxPab polyclonal antibody.

Lane 1: ATP4B transfected lysate(32.12 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ATP4B antibody

Western Blot analysis of ATP4B expression in transfected 293T cell line (H00000496-T02) by ATP4B MaxPab polyclonal antibody.

Lane 1: ATP4B transfected lysate(32.01 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ATP4B antibody

ATP4B MaxPab rabbit polyclonal antibody. Western Blot analysis of ATP4B expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ATP4B antibody

Detection limit for recombinant GST tagged ATP4B is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ATP4B antibody

Transfected 293T; WB Suggested Anti-ATP4B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; ATP4B antibody - middle region (ARP44325_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

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