Antibodies to bcl-2 Protein Family - Product Review 04

Introduction

Bcl-2 and related cytoplasmic proteins are key regulators of apoptosis, the cell suicide program critical for development, tissue homeostasis, and protection against pathogens.

Those most similar to Bcl-2 promote cell survival by inhibiting adapters needed for activation of the proteases (caspases) that dismantle the cell. More distant relatives instead promote apoptosis, apparently through mechanisms that include displacing the adapters from the pro-survival proteins. Thus, for many but not all apoptotic signals, the balance between these competing activities determines cell fate. Bcl-2 family members are essential for maintenance of major organ systems, and mutations affecting them are implicated in cancer.

At least 15 Bcl-2 family members have been identified in mammalian cells and several others in viruses. All members possess at least one of four conserved motifs known as Bcl-2 homology domains (BH1 to BH4). Most pro-survival members, which can inhibit apoptosis in the face of a wide variety of cytotoxic insults, contain at least BH1 and BH2, and those most similar to Bcl-2 have all four BH domains. The two pro-apoptotic subfamilies differ markedly in their relatedness to Bcl-2. Bax, Bak, and Bok (also called Mtd), which contain BH1, BH2, and BH3, resemble Bcl-2 fairly closely. In contrast, the seven other known mammalian "killers" possess only the central short (9 to 16 residue) BH3 domain; they are otherwise unrelated to any known protein, and only Bik and Blk are similar to each other. These "BH3 domain" proteins may well represent the physiological antagonists of the pro-survival proteins.

The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway.





Antibody Tools for Detection of bcl-2 Family Members

Antibody products for detection of many of the above listed bcl-2 family members are available at OriGene Technologies. All antibodies are thoroughly tested for different immunological detection methods. For more informations like host, isotype, species cross-reactivity etc. see table 1 below. For pricing info and datasheet download use the links provided.



Figure 1: Staining of human tonsil using bcl-2 antibody DM130. Note cytoplasmic and memebrane staining of follicular cells. No staining of reactive cells in the germinal center

Figure 1: Staining of human tonsil using bcl-2 antibody DM130. Note cytoplasmic and memebrane staining of follicular cells. No staining of reactive cells in the germinal center

Figure 2:  Staining of Hodgkin lymphoma using bcl-x antibody AM00285PU-N. Note staining of RS cells

Figure 2: Staining of Hodgkin lymphoma using bcl-x antibody AM00285PU-N. Note staining of RS cells

Figure 3:  Staining of Hodgkin lymphoma using bcl-x antibody AP00535PU-N. Note staining of RS cells.

Figure 3: Staining of Hodgkin lymphoma using bcl-x antibody AP00535PU-N. Note staining of RS cells.

Figure 4: Staining of human tonsil using Mcl-1 antibody AM00277PU-N. Note nuclear staining of cells.

Figure 4: Staining of human tonsil using Mcl-1 antibody AM00277PU-N. Note nuclear staining of cells.

Figure 5:  Staining of breast cancer using Bag-1 antibody DM335. Note nuclear and cytoplasmic staining of tumor cells

Figure 5: Staining of breast cancer using Bag-1 antibody DM335. Note nuclear and cytoplasmic staining of tumor cells

Figure 6: Staining of Hodgkin lymphoma using bax antibody DM246. Note membrane staining of RS cells

Figure 6: Staining of Hodgkin lymphoma using bax antibody DM246. Note membrane staining of RS cells

Figure 7: Staining of human tonsil using Bak antibody AP00539PU-N. Note cytoplasmic staining of mucosal epithelial cells.

Figure 7: Staining of human tonsil using Bak antibody AP00539PU-N. Note cytoplasmic staining of mucosal epithelial cells.

Figure 8:  Western blot detection of human bim using SP7027P in K562 (left) and A549 cell lysates (right)

Figure 8: Western blot detection of human bim using SP7027P in K562 (left) and A549 cell lysates (right)

Figure 9: Western blot detection of human bmf using SP1414P in HepG2 (left) and 293 cell lysates (right)

Figure 9: Western blot detection of human bmf using SP1414P in HepG2 (left) and 293 cell lysates (right)

Available antibody panel to bcl-2 Protein Family

Anti- bcl-2, bcl-x, Bcl-xL , Mcl-1, Bag-1, bax, bak, Blk, Bim, Bad, Bmf, NOXA, PUMA-alpha, PUMA beta*



Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

BAG1 (C-term) antibody

Western blot analysis in whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (AP05585PU-N) at 1(A) and 2(B) µg/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €590.00
  OriGene Technologies GmbH

BAG1 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €425.00
  OriGene Technologies GmbH

BAG4 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cerebellum. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

BAG5 antibody

BAG5 Rabbit Purified Rt P, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

Bax antibody

Western blot analysis of Bax expression in rat kidney tissue lysate Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €355.00
  OriGene Technologies GmbH

Bax antibody

Immunohistochemistry in paraffin-embedded human colorectal carcinoma tissue at 1:50 dilution. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2 (61-76) antibody

Bcl-2 Mouse IgG1 10C2 Purified Ms, Rt E, WB
0.1 mg / €300.00
  OriGene Technologies GmbH

Bcl-2 antibody

Western blot analysis of Bcl-2 in HL-60 cell lysate. Rabbit Aff - Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €390.00
  OriGene Technologies GmbH

Bcl-2 pSer70 antibody

Immunohistochemistry analysis of p-BCL-2 (pSer70) Antibody Cat.-No AP01536PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Rt IP, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2 antibody

Bcl-2 Sheep IgG Purified Can, Hu, Rt ICC/IF, IP, WB
0.25 mg / €400.00
  OriGene Technologies GmbH

Bcl-2 antibody

Immunohistochemistry analysis of Bcl-2 antibody (Cat.-No AP06028PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2 antibody

Western blot (WB) analysis of Bcl-2 antibody (Cat.-No.: AP06497PU-N) at 1/500 dilution Lane1: MCF-7 whole cell lysate treated with PMA Lane2: Mouse brain tissue lysate Lane3: Rat kidney tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Bcl-2-like 1 Mouse IgG3 7B2.5 Biotin Hu, Ms, Rt F
0.1 mg / €450.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Bcl-2-like 1 Mouse IgG3 7B2.5 FITC Hu, Ms, Rt F
0.1 mg / €480.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Immunofluorescent Staining. Murine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with Saponin. The cells were incubated with Mouse anti-Human Bcl-xL followed by PE-conjugated Goat anti-Mouse IgG, and then were analyzed by Flow Cytometry. Amount Used: 2 µg/10e6 cells. Mouse IgG3 7B2.5 Purified Hu, Ms, Rt C, F, IP, WB
0.1 mg / €480.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Bcl-2-like 1 Mouse IgG3 7B2.5 PE Hu, Ms, Rt F
0.1 mg / €550.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Western blot analysis of Bcl-xL expression in Jurkat (Lane 1&2), mouse small intestine (Lane 3), and rat kidney (Lane 4) lysates. Rabbit Purified Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL, pSer62) antibody

Bcl-2-like 1 Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Immunohistochemical analysis in paraffin-embedded human breast tissue using Bcl-2-like 1 (Bcl-xL) antibody (Cat.-No. AP06029PU-N). Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Bcl-2-like 1 (Bcl-xL) antibody

Immunohistochemistry (IHC) analyzes of BCL-XL antibody (Cat.-No.: AP06498PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn