Antibodies to cdc2/cdkx Family - Product Review 17

Introduction

The cyclin-dependent kinases (Cdks) are a family of protein kinases whose members are small proteins (~34-40 kD) containing little more than the catalytic core sequences shared by all protein kinases.

They are members of the Ser/Thr protein kinase super family and the cdc2/cdkx subfamily. By definition, all Cdks share the feature that their enzymatic activation requires the binding of a cyclin regulatory subunit. In most cases, full activation also requires phosphorylation of a threonine near the kinase active site. Ckds are turned off by the binding of the CIP or INK4 proteins.

Although originally identified as enzymes that control cell cycle events, members of the Cdk family are involved in other cellular processes as well. Animal cells, for example, contain at least nine Cdks, only four of which (Cdk1, 2, 4, 6) are involved directly in cell cycle control. Another family member (Cdk7) contributes indirectly by acting as a Cdk-activating kinase (CAK) that phosphorylates other Cdks. Cdks are also components of the machinery that controls basal transcription by RNA polymerase II (Cdk7, 8, 9) and are involved in controlling the differentiation of post mitotic neural cells (Cdk5).

Antibody Tools for Detection of Cyclin-dependent Kinases (CDKs)

A full range of polyclonal and monoclonal antibodies is available at Acris Antibodies. Many of these can be used for immunohistochemistry and nearly all for western blotting applications. There is broad inter-species reactivity because Cdk function and structure has been remarkably well conserved during evolution.

Picture Gallery

Staining of human tonsil using cdc2/cdk1 antibody BM2206P clone A17Staining of human tonsil using cdk2 antibody DM341P clone 2B6Figure shows staining of human cervical cancer using anti cdk7 antibody clone MO1.1 cat# BM5019

Fig.1: Formalin-fixed, paraffin-embedded human tonsil stained with cdk1 Ab (Cat.AM00203) using peroxidase-conjugate and DAB chromogen.

Fig.2: Formalin-fixed, paraffin-embedded human tonsil stained with Cdk2 Ab (Cat. AM00247) using peroxidase-conjugate and AEC chromogen.

Fig.3: Paraformaldehyde-fixed human cervical cancer cells (HeLa) stained cdk7 Ab (Cat. BM5019) using peroxidase-conjugate and AEC chromogen.

Figure shows immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antibody (1:500) R1184. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Staining in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: FITC immunofluorescence of mcdk9/PITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain.

Fig.4: Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antibody cat. No. R1184: The staining shows the location of cdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of cdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of cdk9/PITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle.

Available Antibodies to the CDK Family

Anti-
CDK1 (CDC2, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, p34 protein kinase),
CDK2 (Cell division protein kinase 2, cyclin-dependent kinase 2, p33),
CDK3 (Cell division protein kinase 3, cyclin-dependent kinase 3),
CDK4 (Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3),
CDK5 (Cell division protein kinase 5, Cyclin-dependent kinase 5, PSSALRE),
CDK6 (Cell division protein kinase 6, Cyclin-dependent kinase 6, PLSTIRE),
CDK7 (Cell division protein kinase 7, Cyclin-dependent kinase 7, CAK, CAK1, STK1, MO15),
CDK8 (Cell division protein kinase 8, Cyclin-dependent kinase 8, Protein kinase K35),
CDK9 (Cell division protein kinase 9, Cyclin-dependent kinase 9, PITALRE, C-2K, CDC2L4)
antibody

  1. Quick-Filters:
  2. Cited (1)

Items 1 to 20 of 40 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CDK1 antibody

Immunohistochemistry staining of Human thymus (paraffin sections) using anti-Cdk1 (clone POH-1). Mouse IgG2a POH-1 Purified Bov, Hu ICC/IF, IP, P, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

CDK1 antibody

AP15458PU Cdc2 p34 antibody staining of Human Tonsil. Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

CDK1 antibody

AP15458PU Cdc2 p34 antibody staining of Human Tonsil. Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

CDK1 pThr161 antibody

Immunohistochemistry (IHC) analyzes of p-CDC2 (pThr161) antibody Cat.-No.: AP01546PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDK1 pThr161 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using CDC2 (phospho-Thr161) antibody AP02422PU. Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

CDK1 pThr161 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using CDC2 (phospho-Thr161) antibody AP02422PU. Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

CDK1 pTyr15 antibody

Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 antibody (Lane 1, 2, 3 and 4) and CDC2 (pTyr15) antibody (AP02497PU-S; AP02497PU-N, Lane 5, 6, 7 and 8). Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

CDK1 pTyr15 antibody

Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 antibody (Lane 1, 2, 3 and 4) and CDC2 (pTyr15) antibody (AP02497PU-S; AP02497PU-N, Lane 5, 6, 7 and 8). Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

CDK1 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using CDC2 antibody AP02675PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

CDK1 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using CDC2 antibody AP02675PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

CDK1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

CDK1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

CDK1 pTyr15 antibody

CDK1 Rabbit IgG Purified Hu, Ms, Rt, Xen, Ze WB
0.1 ml / €500.00
  Acris Antibodies GmbH

CDK1 antibody

Western blot analysis of extracts from A2780 using CDK1 / CDC2  antibody (Cat.-No. AP06042PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CDK1 pThr161 antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CDK1 antibody

Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CDK1 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 Antibody AP08098PU. Rabbit Aff - Purified Hu, Rt P
0.1 ml / €270.00
  Acris Antibodies GmbH

CDK1 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 Antibody AP08098PU. Rabbit Aff - Purified Hu, Rt P
50 µl / €230.00
  Acris Antibodies GmbH

CDK1 pTyr15 antibody

Figure 1. Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase). These blots are identical to the control except that they were incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Hu, Ms, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

CDK1 pSer39 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Items 1 to 20 of 40 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn