Antibodies to Cytokeratin Proteins - Product Review 09

Introduction

Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells.

Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin 1 (68 kDa) is a secondary type II keratin expressed in cornified epithelia. Cytokeratin 4 (59 kDa) is the secondary type II keratin expressed in non-cornified stratified squamous epithelia.

Cytokeratin 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is the primary type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a ’hyperproliferation‘ cytokeratin expressed in tissues with natural or pathological high turnover. The deduced human cytokeratin 7 protein contains 469 amino acids and has a calculated molecular mass of 51.4 kD. Cytokeratin 7 shares 82% amino acid identity with mouse cytokeratin 7 and 76% identity with rat kangaroo cytokeratin 7.

Western blot analysis of mouse tissues detected cytokeratin 7 expression in bladder, but not in epidermis. Cytokeratin 7 expression was found to be restricted to ducts and specific glands of simple epithelial tissues; examples included isolated cells in the acini of mammary gland, cells of lung alveoli and bronchiolar epithelium, kidney collecting duct, and Brunner glands of the duodenum.

Cytokeratins 10, 13, and 18 are members of the type I acidic subfamily. Cytokeratin 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells.

Cytokeratin 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites. Cytokeratin 20 has an apparent molecular mass of about 46 kD. Antibodies raised against cytokeratin 20 decorated typical cytokeratin fibril arrays in normal and transformed intestinal cells.

Immunocytochemical analysis of several human and rat tissues showed that cytokeratin 20 is a prominent component of intestinal and gastric foveolar epithelium, urothelial umbrella cells, and Merkel cells of epidermis. Sparse positive epithelial cells were noted in the thymus, bronchus, gall bladder, and prostate gland. Monoclonal anti-cytokeratin antibodies are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

Antibody Tools for Detection of Cytokeratins

Acris Antibodies offers one of the worlds most comprehensive antibody product ranges for detection of all important keratins. These antibodies are suitable for immunohistochemical staining procedures (see picture gallery below) and / or other applications like Western blotting.

Picture Gallery

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of human skin using Pan Cytokeratin Marker AE1+AE3 cat# BM5001. Note cytoplasmic staining of epithelial cells

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using pan Cytokeratin marker antibody Lu-5 cat# BM4068. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 20 antibody Ke20.8 cat# DM123. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using acidic Cytokeratin marker antibody AE1 cat# BM5000 Note cytoplasmic staining of tumor cells.

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human prostata using pan basic Cytokeratin marker antibody DE-SQ cat# DM275. Note staining of basal epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human tonsil using anti Cytokeratin 4 antibody 6B10 cat# BM559. Note cytoplasmic staining of mucosal epithelial cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6, 18 antibody LP34 cat# DM051. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human mesothelioma using anti Cytokeratin 5, 6 antibody D5/16B4 cat# DM362. Note cytoplasmic staining of tumor cells

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human lung carcinoma using anti Cytokeratin 5, 8 antibody C50 cat# DM182. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human colon carcinoma using anti Cytokeratin 19 antibody BA-17 cat# SM1822. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human skin using anti Cytokeratin 6 antibody LHK6B cat# DM271. Note cytoplasmic staining of epiderrmal cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human breast carcinoma using anti Cytokeratin 18 antibody DA-7 cat# SM3044. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human ovarian carcinoma using anti Cytokeratin 7 antibody OVTL12/30 cat# DM057. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staining of human skin using anti Cytokeratin 8, 18 antibody 5D3 cat# DM189. Note cytoplasmic staining of epithelial cells in sweat glands

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staing of human lung using anti Cytokeratin 8 antibody M20 cat# BM5045. Note cytoplasmic staining of bronchial epithelial cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human colon carcinoma using anti Cytokeratin 8 antibody 35betaH11 cat# DM054. Note cytoplasmic staining of tumor cells

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 10, 13 antibody DE-K13 cat# DM056. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody LHP1 cat# DM185. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human skin using anti Cytokeratin 10 antibody DE-K10 cat# DM055. Note cytoplasmic staining of suprabasal layers of epithelial cells

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of human tonsil using anti Cytokeratin 13 antibody 1C7 cat# BM5047. Note cytoplasmic staining of mucosal epithelial cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of rat breast carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of squamous cell lung carcinoma using anti Cytokeratin 14 antibody LL002 cat# SM1359. Note cytoplasmic staining of tumor cells.

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staining of human skin using anti Cytokeratin 15 antibody LHK15 cat# DM305. Note cytoplasmic staining of epithelial cells in external rod sheets of hair follicle

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 16 antibody LL025 cat# SM1471. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

Staing of human lung carcinoma using anti Cytokeratin 17 antibody Ks17.E3 cat# DM127. Note cytoplasmic staining of tumor cells.

 

Available Antibody Panel to Cytokeratins

Anti- Cytokeratin (pan), anti- Cytokeratin-1, -3, -4, -5, -6, -7, -8, -9, -10, -13, -14, -15, -16, -17, -18, -19, and -20 antibody

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  
AM10029PU-N

acidic Cytokeratin antibody

acidic Cytokeratin Mouse IgG1 AE1 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
1 ml / €360.00
  Acris Antibodies GmbH
AM10029PU-S

acidic Cytokeratin antibody

acidic Cytokeratin Mouse IgG1 AE1 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
0.5 ml / €270.00
  Acris Antibodies GmbH
AM10030PU-N

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
1 ml / €360.00
  Acris Antibodies GmbH
AM10030PU-S

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Purified Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt C, ICC/IF, P
0.5 ml / €270.00
  Acris Antibodies GmbH
BM5152P

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG2a Ks pan 1-8 Purified Amph, Bov, Hu, Rt C, ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH
GTX20870

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Hu C, ICC/IF, P, WB
6 ml / €320.00
  GeneTex Inc.
MAB2448

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 AE3 Hu C, EM, WB
0.2 ml / €560.00
  Abnova Taiwan Corp.
NB120-850

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG1 34betaE12 Hu C, P
6 ml / €400.00
  Novus Biologicals Inc.
NB120-9287

basic Cytokeratin antibody

basic Cytokeratin Mouse IgG AE3 Aff - Purified Bov, Chk, Hu, Mky, Ms, Rb, Rt C, ICC/IF, P, WB
0.1 mg / €290.00
  Novus Biologicals Inc.
AM05442PU-N

Cytokeratin (1+2+3+4+5+6+7+8) antibody

Cytokeratin (1+2+3+4+5+6+7+8) Mouse IgG2a Ks1-8 Purified Hu C, WB
50 µg / €580.00
  Acris Antibodies GmbH
BM6001P

Cytokeratin (basal cell) antibody

Immunofluorescence staining of a 7 days old Zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs. Mouse IgG1 RCK103 Purified Can, Chk, GP, Hst, Hu, Por, Quail, Rb, Rt, Ze C, F, ICC/IF, WB
0.1 mg / €320.00
  Acris Antibodies GmbH
MAB1829

Cytokeratin (basal cell) antibody

Cytokeratin (basal cell) Mouse IgG1 RCK103 Av, Chk, Hst, Hu, Rb, Rt C, F, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.
MAB1479

Cytokeratin 1 antibody

Cytokeratin 1 Mouse IgG1 IML-91 Hu, Rt C, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.
NB100-2756

Cytokeratin 1 antibody

Immunoctyochemistry: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Cytokeratin 1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue). Mouse LHK1 Purified Hu C, ICC/IF, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.
NB100-2756SS

Cytokeratin 1 antibody

Cytokeratin 1 Mouse IgG2a LHK1 Hu C, ICC/IF, P, WB
25 µl / €200.00
  Novus Biologicals Inc.
NB110-89450

Cytokeratin 1 antibody

Western Blot: CK1 Antibody (PMC01) [NB110-89450] - truncated CK1 recombinant protein. Mouse PMC01 Ascites Hu C, E, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.
GTX16111

Cytokeratin 2e antibody

Immunohistochemical analysis of paraffin embedded human kidney with cytokeratin type II antibody [AE3] Mouse IgG1 AE3 Purified Hu C, EM, WB
0.1 ml / €360.00
  GeneTex Inc.
BM559P

Cytokeratin 4 antibody

Cytokeratin 4 Mouse IgG1 6B10 Purified Can, Fe, Hu, Ze C, F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH
NBP1-42136

Cytokeratin 4 antibody

Cytokeratin 4 Mouse IgG1 6B10 Hu, Rt C, P
5 ml / €1,260.00
  Novus Biologicals Inc.
AM05532PU-N

Cytokeratin 5+6+18 antibody

Cytokeratin 5+6+18 Mouse IgG1 LP34 Hu C
1 ml / €410.00
  Acris Antibodies GmbH

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