Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-1 antibody

Immunoperoxidase of purified MaxPab rabbit antibody to GOT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Rabbit Purified Hu E(detection), P, WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMP-1 antibody

Human Lung; . Rabbit Anti-MMP1 Antibody. . Catalog Number:. ARP42039 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

MMP-1 antibody

Human placenta stained with Anti-MMP-1 antibody Rabbit Purified Hu P, WB
1 ml / €440.00
  Acris Antibodies GmbH

MMP-1 antibody

Human placenta stained with Anti-MMP-1 antibody Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

MMP-1 (active) antibody

Staining of FFPE Human prostate cancer (10x and 40x) with MMP-1 Antibody Cat.-No AF9110 (Clone 195-1H6) at 2 µg/ml. Antibody positive in the cytoplasm of epithelial cells and in stroma. Mouse IgG2b 195-1H6 Purified Hu P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-1 antibody

Left: Immunohistochemical staining using MMP-1 antibody on colon carcinoma tissue section. Right: Western blot using MMP-1 antibody on recombinant human MMP-1 proenzyme (400 ng/lane). Mouse IgG1 3B6 Purified Hu C, WB
0.2 mg / €340.00
  Acris Antibodies GmbH

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e HRP Hu C, E, P, WB
1 ml / €470.00
  Acris Antibodies GmbH

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Purified Hu C, E, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-1 antibody

Western blot (WB) analysis of MMP-1 antibody (Cat.-No.: AP06224PU-N) at 1/500 dilution Lane1:Hela whole cell lysate treated with LPS Lane2:Raw264.7 whole cell lysate treated with LPS Lane3:PC12 whole cell lysate treated with LPS Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MMP-1 (N-term) antibody

Figure 2. Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded Human Prostate tissue using AP07209PU-N at 5 µg/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MMP-1 (hinge region) antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MMP-1 antibody

MMP1 MaxPab polyclonal antibody. Western Blot analysis of MMP1 expression in human pancreas. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMP-1 antibody

Western blot using MMP1 monoclonal antibody, clone 3B6 ( Cat # MAB1950 ) on recombinant human MMP1 proenzyme (400 ng/lane). Hu
0.2 mg / €390.00
  Abnova Taiwan Corp.

MMP-1 antibody

MMP-1 Mouse IgG2b SB12e Hu C, E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MMP-1 antibody

Immunohistochemical staining of MMP1 on formalin fixed, paraffin embedded human placenta with MMP1 polyclonal antibody. Rabbit Hu P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

MMP-1 antibody

Human placenta stained with MMP1 polyclonal antibody ( Cat # PAB12708 ) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Rabbit Hu, Ms, Rt E, IP, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MMP-1 antibody

Human Macrophage; . Sample Type: Human Macrophange Cells. Green: primary. Red: phallodin. Blue: DAPI. Yellow: green/red. Primary. Dilution:.  1:200. Secondary Antibody: anti-Rabbit IgG-FITC. Secondary. Dilution:.  1:1000. Image Submitted By: Milan Fiala. University of California, Los Angeles .; MMP1 antibody - C-terminal region (ARP42040_T100) in Human Macrophage cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Por, Rb WB
0.1 mg / €310.00
  AVIVA Systems Biology

MMP-1 antibody

MMP-1 Rabbit Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

MMP-1 antibody

MMP-1 Mouse IgG1 F15P3B6 Aff - Purified Hu P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MMP-1 antibody

Immunohistochemistry: MMP-1 Antibody (EP1247Y) [NB110-57211] - Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-MMP-1 (cat. #110-57211). Rabbit IgG EP1247Y Aff - Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

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