Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-2 antibody

MMP-2 Rabbit Purified Rt IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

MMP-3 antibody

Staining of Human prostatic adenocarcinoma with MMP-3 Antibody Cat.-No AF6610 (Clone 55-2A4) at 4 µg/ml. Antibody positive in cytoplasma and in lumen secreted. 10x Mouse IgG1 55-2A4 Purified Hu C, E, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-3 antibody

MMP-3 Mouse IgG1 148-1A3 Purified Rb C, E, P, WB

Regular Price: 0.1 mg / €360.00

Special Price: 0.1 mg / €270.00

  Acris Antibodies GmbH

MMP-3 antibody

Left: Immunohistochemical staining of human gastric carcinoma tissue using MMP-3 antibody. Right: Western blot of recombinant human MMP-3 proenzyme using MMP-3 antibody. Mouse IgG1 1B4 Purified Hu E, P, WB
0.2 mg / €340.00
  Acris Antibodies GmbH

MMP-3 antibody

Immunohistochemistry (Paraffin-Embedded Sections): Human colon carcinoma tissue stained with AM08199PU-N at 5 µg/ml. Mouse IgG2a SB14d Purified Hu C, E, P
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €380.00
  Acris Antibodies GmbH

MMP-3 antibody

MMP-3 Rat IgG2 6C34 Purified Ms WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MMP-3 antibody

Western Blot analysis of MMP3 expression in transfected 293T cell line (H00004314-T01) by MMP3 MaxPab polyclonal antibody.

Lane 1: MMP3 transfected lysate(52.47 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMP-3 antibody

MMP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of MMP3 expression in human stomach. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMP-3 antibody

Detection limit for recombinant GST tagged MMP3 is approximately 3ng/ml as a capture antibody. Mouse IgG2b 4C11 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

MMP-3 antibody

Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human MMP-3 Mouse IgG1 MM0021-3E44 Purified Hu P, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

MMP-3 antibody

Human placenta stained with anti-MMP-3, NB 600-1134. Mouse SPM293 Purified Hu ICC/IF, P, WB
0.25 ml / €320.00
  Novus Biologicals Inc.

MMP-3 antibody

Immunohistochemistry: MMP-3 Antibody (SB14d) [NBP1-28615] - 5 ug/mL Paraffin embedded sections of human colon carcinoma were stained with MMP-3. Goat Anti-Mouse IgG2a, human ads-BIOT and Streptavidin-HRP were used and then developed with DAB. Mouse IgG2a SB14d Purified Hu C, E, P
0.1 mg / €400.00
  Novus Biologicals Inc.

MMP-7 antibody

Staining of Human ulcerative gastric adenocarcinoma with MMP-7 Antibody Cat.-No AF8210 (Clone 141-7B2) at 2 µg/ml. Antibody positive in cytoplasma. 40x Mouse IgG1 141-7B2 Purified Hu C, E, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-7 antibody

Staining of FFPE Human colon adenocarcinoma (10x and 40x) with MMP-7 Antibody Cat.-No AF9010 (Clone 176-5F12) at 2 µg/ml. Antibody positive in the cytoplasm of neoplastic epithelial cells and in stroma. Mouse IgG1 176-5F12 Purified Hu P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-7 (132-146) antibody

Figure 1. AP07926PU-N MMP7 antibody staining of Formalin-Fixed Paraffin-Embedded Human Prostate at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

MMP-7 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €380.00
  Acris Antibodies GmbH

MMP-7 antibody

Western Blot analysis of MMP7 expression in transfected 293T cell line (H00004316-T01) by MMP7 MaxPab polyclonal antibody.

Lane 1: MMP7 transfected lysate(29.37 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MMP-7 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MMP7 and FASLG. HeLa cells were stained with anti-MMP7 rabbit purified polyclonal 1:1200 and anti-FASLG mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MMP-7 antibody

Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60988. Mouse IgG2 MM0022-4C21 Purified Hu P, WB
0.1 mg / €450.00
  Novus Biologicals Inc.

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