Antibodies to Matrix Metalloproteinases - Product Review 01

Introduction: Matrix metalloproteinases (MMPs) are a family of more than twenty secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins.

In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterize the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies (see also figure on the right).

 

All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer´s, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

 

Antibody Tools for Detection of Human Matrix Metalloproteinases

Antibody products for detection of most of the above listed MMPs are available at Acris Antibodies.

 

A lot of these antibodies are useful for staining paraffin embedded sections (see picture gallery below).

 

In addition many products can be used for Western blotting procedures, but also immunoprecipitation experiments and ELISA applications are possible.



Table 1: Matrix Metalloproteinases – Overview

Enzyme

Synonym

Mol. Weight*

Main substrates

MMP1

Collagenase I

54 kD

Collagen I, II, III, VII, X

MMP2

Gelatinase A

74 kD

Gelatin I, Collagen VI, V, VII, X

MMP3

Stromelysin 1

54 kD

Degrades Fibronectin, Laminin, Gelatins of type I, III, IV, V; Collagens III, IV, X, IX, cartilage proteoglycans.
Activates procollagenase.

MMP7

Martrilysin

30 kD

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin.
Activates procollagenase.

MMP8

Collagenase II

53 kD

Fibrillar type I, II, and III collagens

MMP9

Gelatinase B

78 kD

Gelatin types I, V and Collagen types IV, V

MMP10

Stromelysin 2

54 kD

Fibronectin, Gelatins of type I, III, IV, V

MMP11

Stromelysin 3

55 kD

Serine protease inhibitors (serpins)

MMP12

Macrophage Elastase

54 kD

Elastin

MMP13

Collagenase III

55 kD

Collagen I

MMP14

MT1-MMP

66 kD

Activates Progelatinase A, degrades many ECM components

MMP15

MT2-MMP

76 kD

Fibronectin, Tenascin, Nidogen, Aggrecan, Perlecan, Laminin

MMP16

MT3-MMP

70 kD

Collagen III, Fibronectin and more

MMP17

MT4-MMP

67 kD

Fibrin and some other ECM components

MMP19

RASI

57 kD

Aggrecan, COMP

MMP20

Enamelysin

54 kD

Amelogenin, Aggrecan, COMP

MMP21

 

65 kD

Alpha-1 Antitrypsin

MMP23

Femalysin

44 kD

Not identified so far. Expressed in ovary, testis.

MMP24

MT5-MMP

73 kD

Activates Progelatinase A, degrades Proteoglycans

MMP25

MT6-MMP

63 kD

Activates Progelatinase A

MMP26

Matrilysin 2

30 kD

Activates Progelatinase B, degrades other ECM components

MMP28

Epilysin

59 kD

Casein

* molecular weight of the unprocessed precursor





Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP1 using AP00545PU-N (paraffin sections). Note cytoplasmic staining of trophoblasts.

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP7 using AM00295PU-N clone ID2 on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP10 using AP00547PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP13 using AM00296PU-N clone VIIIA2 on paraffin embedded sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP14 using AP00550PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of human placenta for MMP16 using AP00552PU-N rabbit polyclonal antibody on paraffin sections

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP7 (MO15014)

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP9 (GT15020).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Staining of paraffin embedded sections using Neuromics* Antibodies to MMP24 (RA15045).

Available antibody panel to Matrix Metalloproteinases

Anti- Matrix Metalloproteinase-1 (MMP-1, Collagenase I), Matrix Metalloproteinase-2 (MMP-2, Gelatinase A), Matrix Metalloproteinase-3 (MMP-3, Stromelysin-1), Matrix Metalloproteinase-7 (MMP-7, Matrilysin) , Matrix Metalloproteinase-8 (MMP-8, Collagenase II), Matrix Metalloproteinase-9 (MMP-9, Gelatinase B), Matrix Metalloproteinase-10 (MMP-10, Stromelysin-2), Matrix Metalloproteinase-11 (MMP-11, Stromelysin-3), Matrix Metalloproteinase-12 (MMP-12, Metalloelastase), Matrix Metalloproteinase-13 (MMP-13, Collagenase III), Matrix Metalloproteinase-14 (MMP-14, MT1-MMP), Matrix Metalloproteinase-15 (MMP-15, MT2-MMP), Matrix Metalloproteinase-16 (MMP-16b, MT3-MMP), Matrix Metalloproteinase-17 (MMP-17, MT4-MMP), Matrix Metalloproteinase-19 (MMP-19, RASI), Matrix Metalloproteinase-20 (MMP-20, Enamelysin), Matrix Metalloproteinase-23 (MMP-23, Femalysin), Matrix Metalloproteinase-23B (MMP-23B), Matrix Metalloproteinase-24 (MMP-24, MT5-MMP), Matrix Metalloproteinase-26 (MMP-26, Matrilysin-2), Matrix Metalloproteinase-28 (MMP-28, Epilysin)



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MMP-9 antibody

Left: Immunohistochemical staining of colon carcinoma using MMP9 antibody. Right: Western blot using MMP9 antibody on recombinant human proenzyme MMP9 (400 ng/lane). Mouse IgG1 2C3 Purified Hu P, WB
0.2 mg / €350.00
  Acris Antibodies GmbH

MMP-9 (Active Protein) antibody

Left: Immunohistochemical staining of oesophageal tumors using Act-MMP9 antibody. Right: Western blot using MMP9 antibody on recombinant human proenzyme MMP9 (left lane) and activated enzyme (right lane). Mouse IgG1 4A3 Purified Hu P, WB
0.2 mg / €350.00
  Acris Antibodies GmbH

MMP-9 antibody

Immunohistochemistry (Paraffin-Embedded Sections): Human colon tissue stained with AM08200PU-N at 5 µg/ml. Mouse IgG2a SB15c Purified Hu C, E, P
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-9 (C-term) antibody

TOP: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 
BUTTOM: Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-9 antibody

10 times concentrated supernatant of porcine endothelial cells. + puromycin, - no puromycin (thanks to Patrick Zani, Institut für Biochemie, Münster) Sheep IgG Purified Hu, Por C, P, WB
1 ml / €500.00
  Acris Antibodies GmbH

MMP-9 antibody

MMP-9 Rabbit Purified Rt C, IP, WB
0.2 mg / €290.00
  Torrey Pines Biolabs

MMP-10 (409-424) antibody

Figure 1. Staining MMP10 in Skin by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue after heat-induced antigen retrieval. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

MMP-10 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-11 (Active protein) antibody

AM08382PU-N MMP-11 antibody staining of Formalin-Fixed Paraffin-Embedded Human Small intestine at 1/200 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 LS3.05 Purified Hu IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MMP-11 (C-term) antibody

Western blot analysis of anti-MMP11 Antibody (C-term) in 293 cell line lysates (35ug/lane). MMP11(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-12 Control Peptide

MMP-12 Purified
50 µg / €180.00
  Acris Antibodies GmbH

MMP-12 (C-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-13 antibody

MMP-13 Mouse IgG1 181-15A12 Purified Hu C, E, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-13 (C-term) antibody

(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-14 / MT1-MMP antibody

Staining of Human gastric fundus with MMP-14 Antibody Cat.-No AF8410 (Clone 113-5B7) at 2 µg/ml. 
Antibody detects citoplasmatic membranous expression of MMP-14  in stromal cells. 10x Mouse IgG3 113-5B7 Purified Hu, Ms, Rb, Rt C, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-14 / MT1-MMP antibody

MMP-14 / MT1-MMP Mouse IgG1 114-1F2 Purified Hu WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-14 / MT1-MMP antibody

Mouse anti-MT1-MMP (Catalog Number AF8610) Staining of breast carcinoma. Mouse IgG1 114-6G6 Purified Hu C, E, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

MMP-14 / MT1-MMP (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MMP-15 antibody

Staining of FFPE Mouse liver HCC (20x and 40x) with MMP15 Antibody Cat.-No AF881 (Clone 162-22G5) at 2 µg/ml. Antibody positive in the cytoplasm of some macrophages and Kuppfer cells of hepatocellular carcinoma (HCC). Mouse IgG1 162-22G5 Purified Ms C, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

MMP-15 (N-term) antibody

The anti-MMP15 N-term Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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