Antibody Panel to Cathepsin Protease Family - Product Review 26

Introduction: The Cathepsins are a group of lysosomal thiol proteinases or endopeptidases found in extracts of various tissues.

Cathepsins, with the exception of Cathepsin S, are active under acidic, but unstable in neutral or alkaline conditions. Cathesin S is active at a neutral pH. The Cathepsins are synthesised by the conversion of a precursor into the mature glycoprotein. Cathepsin activity is regulated by the binding of endogenous protein inhibitors, called cystatins of which Stefin A and B are family members. They have been shown to be important in main physiological and pathological processes and to be of prognostic significance in a number of cancers.

In breast, lung, brain, head and neck cancer a significant prognostic value has been found measuring Cathepsin B activity either by protein assay or immunohistochemical determination. Serum levels of Cathepsin H appeared as prognostic markers for melanoma patients and predicted the effectiveness of the chemoimmunotherapy of these patients. In breast cancer over-expression of Cathepsin L is defined of one of the strongest prognostic factors for a subgroup of patients with node negative breast tumors.

Table 1: Cathepsin Protease Overview

Cathepsin

Molecular Weight* kDa

Cellular Localisation

Tissue Specificity

Function

Cathepsin B

37.8

Lysosomes

no

Thiol Protease

Cathepsin C

51.8

Lysosomes

no

Thiol Protease

Cathepsin D

44.5

Lysosomes

no

Acid Protease

Cathepsin E

43.3

Endosomes

no

Acid Protease

Cathepsin F

53.3

Lysosomes

heart, muscle, brain testis, ovary

Thiol Protease

Cathepsin G

29.0

Membranes

no

Vimentin specific protease

Cathepsin H

37.4

Lysosomes

no

Aminopeptidase

Cathepsin K

37.0

Lysosomes

osteoclasts

Endopeptidase

Cathepsin L

37.5

Lysosomes

no

Thiol Protease

Cathepsin L2/U/V

37.3

Lysosomes

thymus, testis, cornea

Cysteine Protease

Cathepsin O

36.0

Lysosomes

no

Endopeptidase

Cathepsin S

37.5

Lysosomes

no

Thiol Protease

Cathepsin W

42.1

Lysosomes

NK cells,
cytotoxic T-Cells

?

Cathepsin Z

33.9

Lysosomes

no

Carboxydipeptidase

* molecular weight of the unprocessed precursor

Antibody Tools for Detection of Cathepsin Proteases

Antibody products for the detection of most of the above listed Cathepsins are available at Acris Antibodies. These antibodies are suitable for various immunological procedures like immunohistochemistry, ELISA, immunoprecipitation or Western blotting.



Staining of breast carcinoma paraffin sections using anti Cathepsin D monoclonal antibody code BM104

Staining of breast carcinoma paraffin sections using anti Cathepsin D monoclonal antibody code BM104

Staining of human lymph node tissue using anti Cathepsin S monoclonal antibody code SM1669P Intense staining of antigen presenting cells in trabecular and subcapsular sinuses can be seen.

Staining of human lymph node tissue using anti Cathepsin S monoclonal antibody code SM1669P Intense staining of antigen presenting cells in trabecular and subcapsular sinuses can be seen.

Staining of mouse ventral spinal cord using anti Cathepsin D polyclonal antibody code GT15042

Staining of mouse ventral spinal cord using anti Cathepsin D polyclonal antibody code GT15042

 


Literature:

Ref. 1
Obermajer N, Doljak B, Kos J. Cysteine cathepsins: regulators of antitumour immune response. Expert Opin Biol Ther. 2006 Dec;6(12):1295-309.
Ref. 2
Nomura T, Katunuma N. Involvement of cathepsins in the invasion, metastasis and proliferation of cancer cells. J Med Invest. 2005 Feb;52(1-2):1-9.


Available Antibodies to Cathepsin Proteases
Cathepsin B, Cathepsin D, Cathepsin F, Cathepsin G, Cathepsin H, Cathepsin K, Cathepsin L, Cathepsin L2 (U, V), Cathepsin W, Cathepsin Z ? antibody and Protective Protein / Cathepsin A (PPCA) antibody



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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Cathepsin B antibody

Cells were scratch-wounded and stained against cathepsin B and annexin-II heterotetramer subunit p11 after 1 (a), 8 (b) and 24 (c) hours. Fixation was performed with both -20oC cold methanol and acetone for 8 minutes each. Cathepsin B is shown in red, p11 is shown in green. Goat Aff - Purified Ms C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

Cathepsin B Control Peptide

Cathepsin B Purified
50 µg / €190.00
  Acris Antibodies GmbH

Cathepsin B antibody

Western blot analysis of Cathepsin B with Jurkat cell (Lane 1) and mouse small intestine tissue (Lane 2) lysate. Rabbit Purified Hu, Ms, Rt F, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Cathepsin B antibody

Cathepsin B Mouse IgG2b CB131 Supernatant Hu P
1 ml / €530.00
  Acris Antibodies GmbH

Cathepsin B antibody

Cathepsin B Sheep Purified Hu ID, WB
1 ml / €430.00
  Acris Antibodies GmbH

Cathepsin B antibody

Cathepsin B Goat Aff - Purified Hu WB
0.1 mg / €450.00
  Neuromics Antibodies

Cathepsin B antibody

Cathepsin B Mouse IgG2b CB131 Hu P
0.5 ml / €440.00
  Novus Biologicals Inc.

Cathepsin B antibody

ELISA: CATHEPSIN B Antibody [NBP1-21424] Sheep IgG HRP Hu E
0.5 ml / €800.00
  Novus Biologicals Inc.

Cathepsin B antibody

Cathepsin B Sheep FITC Hu E
0.5 ml / €780.00
  Novus Biologicals Inc.

Cathepsin D antibody

Immunohistochemistry: AM09003PU-N Cathepsin D antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b 4G2 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Cathepsin D antibody

Immunohistochemistry: AM09003PU-N Cathepsin D antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b 4G2 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Cathepsin D antibody

Human breast carcinoma stained with anti-cathepsin D antibody Rabbit Purified Hu P
1 ml / €490.00
  Acris Antibodies GmbH

Cathepsin D antibody

Human breast carcinoma stained with anti-cathepsin D antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

Cathepsin D antibody

Cathepsin D Rabbit Purified Hu E, WB
1 ml / €410.00
  Acris Antibodies GmbH

Cathepsin D antibody

Cathepsin D Rabbit Purified Hu C, P
1 ml / €570.00
  Acris Antibodies GmbH

Cathepsin D antibody

Cathepsin D Rabbit Purified Hu C, P
0.5 ml / €380.00
  Acris Antibodies GmbH

Cathepsin D antibody

Cathepsin D Goat Aff - Purified Hu, Ms C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

Cathepsin D antibody

Calbindin D staining of  paraffin-embedded human cerebellum and frozen rat hypothalamus sections using 25 µg/mL mouse anti-human Calbindin D monoclonal antibody. Tissues were stained using anti-mouse HRP-DAB ( brown) and counterstained with hematoxylin (blue). Mouse IgG - Purified IP
0.1 mg / €340.00
  Neuromics Antibodies

Cathepsin D antibody

ELISA: Cathepsin D Antibody [22580002] Rabbit Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

Cathepsin D antibody

Cathepsin D Rabbit IgG Purified Hu P
0.25 ml / €340.00
  Novus Biologicals Inc.

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